{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liu J"],"funding":["Ministry of Education - Singapore"],"pagination":["7266-7273"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10959107"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["146(11)"],"pubmed_abstract":["Tension gauge tethers (TGTs), short DNA segments serving as extracellular tension sensors, are instrumental in assessing the tension dynamics in mechanotransduction. These TGTs feature an initial shear-stretch region and an unzip-stretch region. Despite their utility, no theoretical model has been available to estimate their tension-dependent lifetimes [τ(<i>f</i>)], restricting insights from cellular mechanotransduction experiments. We have now formulated a concise expression for τ(<i>f</i>) of TGTs, accommodating contributions from both stretch regions. Our model uncovers a tension-dependent energy barrier shift occurring when tension surpasses a switching force of approximately 13 pN for the recently developed TGTs, greatly influencing τ(<i>f</i>) profiles. Experimental data from several TGTs validated our model. The calibrated expression can predict τ(<i>f</i>) of TGTs at 37 °C based on their sequences with minor fold changes, supporting future applications of TGTs."],"journal":["Journal of the American Chemical Society"],"pubmed_title":["Unraveling the Dual-Stretch-Mode Impact on Tension Gauge Tethers' Mechanical Stability."],"pmcid":["PMC10959107"],"funding_grant_id":["MOE-T2EP50220-0015","MOE-T2EP50123-0008","MOET32021-0003"],"pubmed_authors":["Liu J","Yan J"],"additional_accession":[]},"is_claimable":false,"name":"Unraveling the Dual-Stretch-Mode Impact on Tension Gauge Tethers' Mechanical Stability.","description":"Tension gauge tethers (TGTs), short DNA segments serving as extracellular tension sensors, are instrumental in assessing the tension dynamics in mechanotransduction. These TGTs feature an initial shear-stretch region and an unzip-stretch region. Despite their utility, no theoretical model has been available to estimate their tension-dependent lifetimes [τ(<i>f</i>)], restricting insights from cellular mechanotransduction experiments. We have now formulated a concise expression for τ(<i>f</i>) of TGTs, accommodating contributions from both stretch regions. Our model uncovers a tension-dependent energy barrier shift occurring when tension surpasses a switching force of approximately 13 pN for the recently developed TGTs, greatly influencing τ(<i>f</i>) profiles. Experimental data from several TGTs validated our model. The calibrated expression can predict τ(<i>f</i>) of TGTs at 37 °C based on their sequences with minor fold changes, supporting future applications of TGTs.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-04T02:45:28.216Z","creation":"2025-04-04T02:45:28.216Z"},"accession":"S-EPMC10959107","cross_references":{"pubmed":["38451494"],"doi":["10.1021/jacs.3c10923"]}}