{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kelly CM"],"funding":["NHLBI NIH HHS","NIGMS NIH HHS"],"pagination":["88-98"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10959293"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["185"],"pubmed_abstract":["<h4>Rationale</h4>Cardiac muscle cells are terminally differentiated after birth and must beat continually throughout one's lifetime. This mechanical process is driven by the sliding of actin-based thin filaments along myosin-based thick filaments, organized within sarcomeres. Despite costly energetic demand, the half-life of the proteins that comprise the cardiac thick filaments is ∼10 days, with individual molecules being replaced stochastically, by unknown mechanisms.<h4>Objectives</h4>To allow for the stochastic replacement of molecules, we hypothesized that the structure of thick filaments must be highly dynamic in vivo.<h4>Methods and results</h4>To test this hypothesis in adult mouse hearts, we replaced a fraction of the endogenous myosin regulatory light chain (RLC), a component of"],"journal":["Journal of molecular and cellular cardiology"],"pubmed_title":["Visualization of cardiac thick filament dynamics in ex vivo heart preparations."],"pmcid":["PMC10959293"],"funding_grant_id":["R01 HL157487","T32 HL076122","P20 GM135007"],"pubmed_authors":["Previs MJ","Kelly CM","Martin JL","Coseno M"],"additional_accession":[]},"is_claimable":false,"name":"Visualization of cardiac thick filament dynamics in ex vivo heart preparations.","description":"<h4>Rationale</h4>Cardiac muscle cells are terminally differentiated after birth and must beat continually throughout one's lifetime. This mechanical process is driven by the sliding of actin-based thin filaments along myosin-based thick filaments, organized within sarcomeres. Despite costly energetic demand, the half-life of the proteins that comprise the cardiac thick filaments is ∼10 days, with individual molecules being replaced stochastically, by unknown mechanisms.<h4>Objectives</h4>To allow for the stochastic replacement of molecules, we hypothesized that the structure of thick filaments must be highly dynamic in vivo.<h4>Methods and results</h4>To test this hypothesis in adult mouse hearts, we replaced a fraction of the endogenous myosin regulatory light chain (RLC), a component of","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Dec","modification":"2025-04-19T16:09:57.946Z","creation":"2025-04-19T16:09:57.946Z"},"accession":"S-EPMC10959293","cross_references":{"pubmed":["37923195"],"doi":["10.1016/j.yjmcc.2023.10.013"]}}