biostudies-literatureUnknownMandler MDRNA modifications have a substantial impact on tRNA function, with modifications in the anticodon loop contributing to translational fidelity and modifications in the tRNA core impacting structural stability. In bacteria, tRNA modifications are crucial for responding to stress and regulating the expression of virulence factors. Although tRNA modifications are well-characterized in a few model organisms, our knowledge of tRNA modifications in human pathogens, such as <i>Pseudomonas aeruginosa</i>, remains limited. Here we leveraged two orthogonal approaches to build a reference landscape of tRNA modifications in <i>E. coli</i>, which enabled us to identify similar modifications in <i>P. aeruginosa</i>. Our analysis revealed a substantial degree of conservation between the two organisms, whibioRxiv : the preprint server for biology2024.02.21.581370https://www.ebi.ac.uk/biostudies/studies/S-EPMC10962704biostudies-literatureThe modification landscape of <i>P. aeruginosa</i> tRNAs.PMC10962704Guiblet WMWarrell DLFitzsimmons CMMandler MDMaligireddy SSBatista PJMcDonald KSfalseThe modification landscape of <i>P. aeruginosa</i> tRNAs.RNA modifications have a substantial impact on tRNA function, with modifications in the anticodon loop contributing to translational fidelity and modifications in the tRNA core impacting structural stability. In bacteria, tRNA modifications are crucial for responding to stress and regulating the expression of virulence factors. Although tRNA modifications are well-characterized in a few model organisms, our knowledge of tRNA modifications in human pathogens, such as <i>Pseudomonas aeruginosa</i>, remains limited. Here we leveraged two orthogonal approaches to build a reference landscape of tRNA modifications in <i>E. coli</i>, which enabled us to identify similar modifications in <i>P. aeruginosa</i>. Our analysis revealed a substantial degree of conservation between the two organisms, whi2024-01-01T00:00:00Z2024 Feb2026-04-08T15:26:28.7Z2025-04-05T23:16:22.877ZS-EPMC109627043852950810.1101/2024.02.21.581370