{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Dashti P"],"funding":["NIAMS NIH HHS"],"pagination":["116866"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10962865"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["176"],"pubmed_abstract":["Osteoblast differentiation is epigenetically suppressed by the H3K27 methyltransferase EZH2, and induced by the morphogen BMP2 and transcription factor RUNX2. These factors also regulate distinct G protein coupled receptors (GPRCs; e.g., PTH1R, GPR30/GPER1). Because GPRCs transduce many physiological stimuli, we examined whether BMP2 or EZH2 inhibition (i.e., GSK126) regulates other GPRC genes in osteoblasts. RNA-seq screening of >400 mouse GPRC-related genes showed that many GPRCs are downregulated during osteogenic differentiation. The orphan receptor GPRC5C, along with a small subset of other GPRCs, is induced by BMP2 or GSK126 during Vitamin C dependent osteoblast differentiation, but not by all-trans retinoic acid. ChIP-seq analysis revealed that GSK126 reduces H3K27me3 levels at the "],"journal":["Bone"],"pubmed_title":["G-protein coupled receptor 5C (GPRC5C) is required for osteoblast differentiation and responds to EZH2 inhibition and multiple osteogenic signals."],"pmcid":["PMC10962865"],"funding_grant_id":["R01 AR049069"],"pubmed_authors":["van der Eerden BJ","Thaler R","Hawse JR","Galvan ML","van Wijnen AJ","Dudakovic A","Dashti P"],"additional_accession":[]},"is_claimable":false,"name":"G-protein coupled receptor 5C (GPRC5C) is required for osteoblast differentiation and responds to EZH2 inhibition and multiple osteogenic signals.","description":"Osteoblast differentiation is epigenetically suppressed by the H3K27 methyltransferase EZH2, and induced by the morphogen BMP2 and transcription factor RUNX2. These factors also regulate distinct G protein coupled receptors (GPRCs; e.g., PTH1R, GPR30/GPER1). Because GPRCs transduce many physiological stimuli, we examined whether BMP2 or EZH2 inhibition (i.e., GSK126) regulates other GPRC genes in osteoblasts. RNA-seq screening of >400 mouse GPRC-related genes showed that many GPRCs are downregulated during osteogenic differentiation. The orphan receptor GPRC5C, along with a small subset of other GPRCs, is induced by BMP2 or GSK126 during Vitamin C dependent osteoblast differentiation, but not by all-trans retinoic acid. ChIP-seq analysis revealed that GSK126 reduces H3K27me3 levels at the ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2026-06-03T07:21:09.661Z","creation":"2025-04-06T09:33:48.093Z"},"accession":"S-EPMC10962865","cross_references":{"pubmed":["37558192"],"doi":["10.1016/j.bone.2023.116866"]}}