{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Song T"],"funding":["National Institute of Allergy and Infectious Diseases","NIAID NIH HHS"],"pagination":["5894-5900"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10965196"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["146(9)"],"pubmed_abstract":["Coronavirus transmission and mutations have brought intensive challenges on pandemic control and disease treatment. Developing robust and versatile antiviral drugs for viral neutralization is highly desired. Here, we created a new polyvalent nanobody (Nb) structure that shows the effective inhibition of SARS-CoV-2 infections. Our polyvalent Nb structure, called \"PNS\", is achieved by first conjugating single-stranded DNA (ssDNA) and the receptor-binding domain (RBD)-targeting Nb with retained binding ability to SARS-CoV-2 spike protein and then coalescing the ssDNA-Nb conjugates around a gold nanoparticle (AuNP) via DNA hybridization with a desired Nb density that offers spatial pattern-matching with that of the Nb binding sites on the trimeric spike. The surface plasmon resonance (SPR) ass"],"journal":["Journal of the American Chemical Society"],"pubmed_title":["Polyvalent Nanobody Structure Designed for Boosting SARS-CoV-2 Inhibition."],"pmcid":["PMC10965196"],"funding_grant_id":["R01 AI159454","R21AI166898","R21 AI166898","R01AI159454"],"pubmed_authors":["Cooper L","Song T","Dwivedy A","Wang X","Galvan Achi J","Rong L"],"additional_accession":[]},"is_claimable":false,"name":"Polyvalent Nanobody Structure Designed for Boosting SARS-CoV-2 Inhibition.","description":"Coronavirus transmission and mutations have brought intensive challenges on pandemic control and disease treatment. Developing robust and versatile antiviral drugs for viral neutralization is highly desired. Here, we created a new polyvalent nanobody (Nb) structure that shows the effective inhibition of SARS-CoV-2 infections. Our polyvalent Nb structure, called \"PNS\", is achieved by first conjugating single-stranded DNA (ssDNA) and the receptor-binding domain (RBD)-targeting Nb with retained binding ability to SARS-CoV-2 spike protein and then coalescing the ssDNA-Nb conjugates around a gold nanoparticle (AuNP) via DNA hybridization with a desired Nb density that offers spatial pattern-matching with that of the Nb binding sites on the trimeric spike. The surface plasmon resonance (SPR) ass","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-18T21:20:25.45Z","creation":"2025-04-07T09:17:08.602Z"},"accession":"S-EPMC10965196","cross_references":{"pubmed":["38408177"],"doi":["10.1021/jacs.3c11760"]}}