biostudies-literatureYu RCMinistry of Science and Technology of the People's Republic of China (Chinese Ministry of Science and Technology)the Fundamental Research Funds for the Central UniversitiesMinistry of Science and Technology of the People's Republic of China2654https://www.ebi.ac.uk/biostudies/studies/S-EPMC10966104biostudies-literatureUnknown15(1)The Myoviridae cyanophage A-1(L) specifically infects the model cyanobacteria Anabaena sp. PCC 7120. Following our recent report on the capsid structure of A-1(L), here we present the high-resolution cryo-EM structure of its intact tail machine including the neck, tail and attached fibers. Besides the dodecameric portal, the neck contains a canonical hexamer connected to a unique pentadecamer that anchors five extended bead-chain-like neck fibers. The 1045-Å-long contractile tail is composed of a helical bundle of tape measure proteins surrounded by a layer of tube proteins and a layer of sheath proteins, ended with a five-component baseplate. The six long and six short tail fibers are folded back pairwise, each with one end anchoring to the baseplate and the distal end pointing to the capNature communicationsStructure of the intact tail machine of Anabaena myophage A-1(L).PMC109661042018YFA0903100Zhang HYCui NHou PZhu JZhou CZChen YLi QDu KXu XYu RCYang FfalseStructure of the intact tail machine of Anabaena myophage A-1(L).The Myoviridae cyanophage A-1(L) specifically infects the model cyanobacteria Anabaena sp. PCC 7120. Following our recent report on the capsid structure of A-1(L), here we present the high-resolution cryo-EM structure of its intact tail machine including the neck, tail and attached fibers. Besides the dodecameric portal, the neck contains a canonical hexamer connected to a unique pentadecamer that anchors five extended bead-chain-like neck fibers. The 1045-Å-long contractile tail is composed of a helical bundle of tape measure proteins surrounded by a layer of tube proteins and a layer of sheath proteins, ended with a five-component baseplate. The six long and six short tail fibers are folded back pairwise, each with one end anchoring to the baseplate and the distal end pointing to the cap2024-01-01T00:00:00Z2024 Mar2026-07-16T00:19:32.768Z2024-12-03T23:57:19.564ZS-EPMC109661043853197210.1038/s41467-024-47006-z