{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7"],"submitter":["Tabassum N"],"pubmed_abstract":["The increasing incidence of antimicrobial resistance exhibited by biofilm-forming microbial pathogens has been recognized as one of the major issues in the healthcare sector. In the present study, nanomaterial-based controlling the biofilm and virulence properties has been considered an alternative approach. Pyoverdine (PVD) isolated from the <i>Pseudomonas aeruginosa</i> was utilized as a biological corona to synthesize silver nanoparticles (AgNPs), which will be helpful in a targeted action to microbial pathogens due to the recognition of the corona of the nanoparticles by the pathogenic membrane. Synthesized PVD-AgNPs were spherical to irregular, with an average size value of 251.87 ± 21.8 nm and zeta potential with a value of -36.51 ± 0.69 mV. The MIC value of PVD-AgNPs towards <i>P. aeruginosa</i>, <i>Listeria monocytogenes</i>, <i>Staphylococcus aureus</i>, <i>Streptococcus mutans</i>, <i>Escherichia coli</i>, and <i>Candida albicans</i> in the standard and host-mimicking media were observed in decreasing order in a multi-fold, such as standard growth media > sputum > synthetic human urine > saliva. Both the initial stage and the well-established biofilms of these microbial pathogens have been effectively inhibited and eradicated by PVD-AgNPs. PVD-AgNPs increase the susceptibility of tetracycline, PVD, and amphotericin B towards established mature mono- and mixed-species biofilms of <i>S. aureus</i> and <i>C. albicans</i>. Additionally, PVD-AgNPs attenuate several virulence properties, such as inhibition of protease activity, motility, and PVD and pyocyanin production in <i>P. aeruginosa</i>. The inhibition of gene expression of biofilm and virulence-associated genes in <i>P. aeruginosa</i> validates its phenotypic effects."],"journal":["Biofilm"],"pagination":["100192"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10966193"],"repository":["biostudies-literature"],"pubmed_title":["Silver nanoparticles synthesized from <i>Pseudomonas aeruginosa</i> pyoverdine: Antibiofilm and antivirulence agents."],"pmcid":["PMC10966193"],"pubmed_authors":["Kim YM","Jo DM","Khan F","Jeong GJ","Tabassum N"],"additional_accession":[]},"is_claimable":false,"name":"Silver nanoparticles synthesized from <i>Pseudomonas aeruginosa</i> pyoverdine: Antibiofilm and antivirulence agents.","description":"The increasing incidence of antimicrobial resistance exhibited by biofilm-forming microbial pathogens has been recognized as one of the major issues in the healthcare sector. In the present study, nanomaterial-based controlling the biofilm and virulence properties has been considered an alternative approach. Pyoverdine (PVD) isolated from the <i>Pseudomonas aeruginosa</i> was utilized as a biological corona to synthesize silver nanoparticles (AgNPs), which will be helpful in a targeted action to microbial pathogens due to the recognition of the corona of the nanoparticles by the pathogenic membrane. Synthesized PVD-AgNPs were spherical to irregular, with an average size value of 251.87 ± 21.8 nm and zeta potential with a value of -36.51 ± 0.69 mV. The MIC value of PVD-AgNPs towards <i>P. aeruginosa</i>, <i>Listeria monocytogenes</i>, <i>Staphylococcus aureus</i>, <i>Streptococcus mutans</i>, <i>Escherichia coli</i>, and <i>Candida albicans</i> in the standard and host-mimicking media were observed in decreasing order in a multi-fold, such as standard growth media > sputum > synthetic human urine > saliva. Both the initial stage and the well-established biofilms of these microbial pathogens have been effectively inhibited and eradicated by PVD-AgNPs. PVD-AgNPs increase the susceptibility of tetracycline, PVD, and amphotericin B towards established mature mono- and mixed-species biofilms of <i>S. aureus</i> and <i>C. albicans</i>. Additionally, PVD-AgNPs attenuate several virulence properties, such as inhibition of protease activity, motility, and PVD and pyocyanin production in <i>P. aeruginosa</i>. The inhibition of gene expression of biofilm and virulence-associated genes in <i>P. aeruginosa</i> validates its phenotypic effects.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2025-04-04T23:52:56.738Z","creation":"2025-04-04T23:52:56.738Z"},"accession":"S-EPMC10966193","cross_references":{"pubmed":["38544742"],"doi":["10.1016/j.bioflm.2024.100192"]}}