{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tomris I"],"funding":["European Commission","NWO Rubicon Grant"],"pagination":["e4974"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10966353"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["33(4)"],"pubmed_abstract":["Enveloped viruses carry one or multiple proteins with receptor-binding functionalities. Functional receptors can be glycans, proteinaceous, or both; therefore, recombinant protein approaches are instrumental in attaining new insights regarding viral envelope protein receptor-binding properties. Visualizing and measuring receptor binding typically entails antibody detection or direct labeling, whereas direct fluorescent fusions are attractive tools in molecular biology. Here, we report a suite of distinct fluorescent fusions, both N- and C-terminal, for influenza A virus hemagglutinins and SARS-CoV-2 spike RBD. The proteins contained three or six fluorescent protein barrels and were applied directly to cells to assess receptor binding properties."],"journal":["Protein science : a publication of the Protein Society"],"pubmed_title":["Viral envelope proteins fused to multiple distinct fluorescent reporters to probe receptor binding."],"pmcid":["PMC10966353"],"funding_grant_id":["45219118","802780"],"pubmed_authors":["van der Woude R","de Paiva Froes Rocha R","de Vries RP","Tomris I","Torrents de la Pena A","Ward AB"],"additional_accession":[]},"is_claimable":false,"name":"Viral envelope proteins fused to multiple distinct fluorescent reporters to probe receptor binding.","description":"Enveloped viruses carry one or multiple proteins with receptor-binding functionalities. Functional receptors can be glycans, proteinaceous, or both; therefore, recombinant protein approaches are instrumental in attaining new insights regarding viral envelope protein receptor-binding properties. Visualizing and measuring receptor binding typically entails antibody detection or direct labeling, whereas direct fluorescent fusions are attractive tools in molecular biology. Here, we report a suite of distinct fluorescent fusions, both N- and C-terminal, for influenza A virus hemagglutinins and SARS-CoV-2 spike RBD. The proteins contained three or six fluorescent protein barrels and were applied directly to cells to assess receptor binding properties.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-06-03T06:52:11.159Z","creation":"2025-04-04T22:59:32.035Z"},"accession":"S-EPMC10966353","cross_references":{"pubmed":["38533540"],"doi":["10.1002/pro.4974"]}}