biostudies-literatureTomris IEuropean CommissionNWO Rubicon Grante4974https://www.ebi.ac.uk/biostudies/studies/S-EPMC10966353biostudies-literatureUnknown33(4)Enveloped viruses carry one or multiple proteins with receptor-binding functionalities. Functional receptors can be glycans, proteinaceous, or both; therefore, recombinant protein approaches are instrumental in attaining new insights regarding viral envelope protein receptor-binding properties. Visualizing and measuring receptor binding typically entails antibody detection or direct labeling, whereas direct fluorescent fusions are attractive tools in molecular biology. Here, we report a suite of distinct fluorescent fusions, both N- and C-terminal, for influenza A virus hemagglutinins and SARS-CoV-2 spike RBD. The proteins contained three or six fluorescent protein barrels and were applied directly to cells to assess receptor binding properties.Protein science : a publication of the Protein SocietyViral envelope proteins fused to multiple distinct fluorescent reporters to probe receptor binding.PMC1096635345219118802780van der Woude Rde Paiva Froes Rocha Rde Vries RPTomris ITorrents de la Pena AWard ABfalseViral envelope proteins fused to multiple distinct fluorescent reporters to probe receptor binding.Enveloped viruses carry one or multiple proteins with receptor-binding functionalities. Functional receptors can be glycans, proteinaceous, or both; therefore, recombinant protein approaches are instrumental in attaining new insights regarding viral envelope protein receptor-binding properties. Visualizing and measuring receptor binding typically entails antibody detection or direct labeling, whereas direct fluorescent fusions are attractive tools in molecular biology. Here, we report a suite of distinct fluorescent fusions, both N- and C-terminal, for influenza A virus hemagglutinins and SARS-CoV-2 spike RBD. The proteins contained three or six fluorescent protein barrels and were applied directly to cells to assess receptor binding properties.2024-01-01T00:00:00Z2024 Apr2026-06-03T06:52:11.159Z2025-04-04T22:59:32.035ZS-EPMC109663533853354010.1002/pro.4974