biostudies-literatureUnknown8Li YCurrently, dairy mastitis caused by <i>Staphylococcus xylosus</i> poses a serious challenge for dairy farming. In this study, we explored the role and mechanism of rhein against <i>S. xylosus</i> with the hope of providing new research ideas to solve mastitis in dairy cows and ensure the source safety of dairy products. Through in vitro antimicrobial studies, we found that the minimum inhibitory concentration (MIC) of rhein was 64 μg/mL, and it significantly interfered with the formation of <i>S. xylosus</i> biofilm at sub-MIC. In experiments on mastitis in mice, rhein alleviated inflammation in mammary tissue, reduced the levels of TNF-α and IL-6, and decreased the number of <i>S. xylosus</i>. To explore the anti-<i>S. xylosus</i> mechanism of rhein, we identified the relevant proteins inCurrent research in food science100718https://www.ebi.ac.uk/biostudies/studies/S-EPMC10966458biostudies-literatureRhein against <i>Staphylococcus xylosus</i> by interfering with respiratory metabolism and inducing oxidative stress.PMC10966458Li GDing WLi YTang LChen JZhang ZHuang GChen WMa JHe QKong XfalseRhein against <i>Staphylococcus xylosus</i> by interfering with respiratory metabolism and inducing oxidative stress.Currently, dairy mastitis caused by <i>Staphylococcus xylosus</i> poses a serious challenge for dairy farming. In this study, we explored the role and mechanism of rhein against <i>S. xylosus</i> with the hope of providing new research ideas to solve mastitis in dairy cows and ensure the source safety of dairy products. Through in vitro antimicrobial studies, we found that the minimum inhibitory concentration (MIC) of rhein was 64 μg/mL, and it significantly interfered with the formation of <i>S. xylosus</i> biofilm at sub-MIC. In experiments on mastitis in mice, rhein alleviated inflammation in mammary tissue, reduced the levels of TNF-α and IL-6, and decreased the number of <i>S. xylosus</i>. To explore the anti-<i>S. xylosus</i> mechanism of rhein, we identified the relevant proteins in2024-01-01T00:00:00Z20242025-04-21T21:29:57.111Z2025-04-05T18:23:05.379ZS-EPMC109664583854537810.1016/j.crfs.2024.100718