biostudies-literatureLei MU.S. National Science FoundationThe Shanghai Sailing Program111 ProjectThe Shanghai Postdoctoral Excellence Programthe Betty and Gordon Moore FoundationNational Natural Science Foundation of ChinaChina Postdoctoral Science FoundationNational Postdoctoral Program for Innovative TalentsHigher Education Discipline Innovation ProjectNational Key Research and Development Program of ChinaNational Science FoundationScience and Technology Innovation Project and Excellent Academic Leader Project of Shanghai Science and Technology Committeee2307606https://www.ebi.ac.uk/biostudies/studies/S-EPMC10966538biostudies-literatureUnknown11(12)Biology remains the envy of flexible soft matter fabrication because it can satisfy multiple functional needs by organizing a small set of proteins and polysaccharides into hierarchical systems with controlled heterogeneity in composition and microstructure. Here, it is reported that controlled, mild electronic inputs (<10 V; <20 min) induce a homogeneous gelatin-chitosan mixture to undergo sorting and bottom-up self-assembly into a Janus film with compositional gradient (i.e., from chitosan-enriched layer to chitosan/gelatin-contained layer) and tunable dense-porous gradient microstructures (e.g., porosity, pore size, and ratio of dense to porous layers). This Janus film performs is shown multiple functions for guided bone regeneration: the integration of compositional and microstructuralAdvanced science (Weinheim, Baden-Wurttemberg, Germany)Electro-Sorting Create Heterogeneity: Constructing A Multifunctional Janus Film with Integrated Compositional and Microstructural Gradients for Guided Bone Regeneration.PMC10966538BX202301221139523YF14097003230111332171341202215721XD1421100CBET‐19329632021YFB3800800CBET-19329632022M721136B1401821S3190150031922041Payne GFLei MZhu JLiao HWang SQu XWan HLiu CZhou HfalseElectro-Sorting Create Heterogeneity: Constructing A Multifunctional Janus Film with Integrated Compositional and Microstructural Gradients for Guided Bone Regeneration.Biology remains the envy of flexible soft matter fabrication because it can satisfy multiple functional needs by organizing a small set of proteins and polysaccharides into hierarchical systems with controlled heterogeneity in composition and microstructure. Here, it is reported that controlled, mild electronic inputs (<10 V; <20 min) induce a homogeneous gelatin-chitosan mixture to undergo sorting and bottom-up self-assembly into a Janus film with compositional gradient (i.e., from chitosan-enriched layer to chitosan/gelatin-contained layer) and tunable dense-porous gradient microstructures (e.g., porosity, pore size, and ratio of dense to porous layers). This Janus film performs is shown multiple functions for guided bone regeneration: the integration of compositional and microstructural2024-01-01T00:00:00Z2024 Mar2025-04-27T03:09:47.171Z2025-04-27T03:09:47.171ZS-EPMC109665383822569710.1002/advs.202307606