biostudies-literatureBallaben ASConselho Nacional de Desenvolvimento Científico e TecnológicoNIAID NIH HHSCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorFundação de Amparo à Pesquisa do Estado de São PauloNational Institutes of Health104658https://www.ebi.ac.uk/biostudies/studies/S-EPMC10966726biostudies-literatureUnknown87IMP-1-producing Pseudomonas aeruginosa was first reported in Japan and since then, bacteria with this metallo-β-lactamase have been detected worldwide. Pseudomonas monteilii (part of P. putida group) were considered an environmental pathogen with low virulence potential; however, multidrug-resistant and carbapenem-resistant P. monteilii have emerged. The present study reports the draft sequence of an extensively drug-resistant IMP-16-producing P. monteilii 597/14 isolated from cerebrospinal fluid in 2014. The sequencing data revealed bla_IMP-16 as a gene cassette on class 1 integron, In1738 characterized in this study. Furthermore, the resistome of Pm597/14 consisted of 7 resistance genes (aadA1b, strA, strB, aacA4, bla_IMP-16, bla_OXA-2, sul1) and diverse virulence determinants involved in the adherence, LPS, antiphagocytosis, iron uptake and mercuric resistance. Although different virulence determinants were found in this study, using Galleria mellonella infection model, Pm597/14 did not kill any larvae between 7 days post-infection. P. monteilii isolates have been reported from clinical and environmental sources, carrying different MBL genes showing its potential role as their reservoir.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseasesExtensively drug-resistant IMP-16-producing Pseudomonas monteilii isolated from cerebrospinal fluid.PMC10966726R21AI1355222015/23484–9R01 AI104895R01AI1048952015/11728–02014/14494–8R21 AI135522Ferreira JCAndrade LNde Oliveira Garcia DDoi YDarini ALCGaletti RBallaben ASfalseExtensively drug-resistant IMP-16-producing Pseudomonas monteilii isolated from cerebrospinal fluid.IMP-1-producing Pseudomonas aeruginosa was first reported in Japan and since then, bacteria with this metallo-β-lactamase have been detected worldwide. Pseudomonas monteilii (part of P. putida group) were considered an environmental pathogen with low virulence potential; however, multidrug-resistant and carbapenem-resistant P. monteilii have emerged. The present study reports the draft sequence of an extensively drug-resistant IMP-16-producing P. monteilii 597/14 isolated from cerebrospinal fluid in 2014. The sequencing data revealed bla_IMP-16 as a gene cassette on class 1 integron, In1738 characterized in this study. Furthermore, the resistome of Pm597/14 consisted of 7 resistance genes (aadA1b, strA, strB, aacA4, bla_IMP-16, bla_OXA-2, sul1) and diverse virulence determinants involved in the adherence, LPS, antiphagocytosis, iron uptake and mercuric resistance. Although different virulence determinants were found in this study, using Galleria mellonella infection model, Pm597/14 did not kill any larvae between 7 days post-infection. P. monteilii isolates have been reported from clinical and environmental sources, carrying different MBL genes showing its potential role as their reservoir.2021-01-01T00:00:00Z2021 Jan2025-04-21T21:30:24.497Z2025-04-05T18:22:17.345ZS-EPMC109667263327133710.1016/j.meegid.2020.104658