{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wu G"],"funding":["the Technology System of Bulk Vegetable Industry in Henan Province","the Tackle Project in Science and Technology of Henan Province"],"pagination":["323"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10967493"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(3)"],"pubmed_abstract":["Tomato is the vegetable with the largest greenhouse area in China, and low temperature is one of the main factors affecting tomato growth, yield, and quality. Hydrogen sulfide (H<sub>2</sub>S) plays an important role in regulating plant chilling tolerance, but its downstream cascade reaction and mechanism remain unclear. Mitogen-activated protein kinases (MAPK/MPKs) are closely related to a variety of signaling substances in stress signal transmission. However, whether H<sub>2</sub>S is related to the MPK cascade pathway in response to low-temperature stress is rarely reported. In this study, NaHS treatment significantly decreased the electrolyte leakage (EL), superoxide anion (O<sub>2</sub><sup>-</sup>) production rate, and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) content of seedlings at low temperatures. In addition, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were obviously increased; and the photochemical efficiency of PSII (Fv/Fm) was enhanced with treatment with NaHS, indicating that NaHS improved the seedlings' cold tolerance by alleviating the degree of membrane lipid peroxidation and oxidative damage. However, H<sub>2</sub>S scavenger hypotaurine (HT) treatment showed the opposite effect. We found that H<sub>2</sub>S content, L-cysteine desulfhydrase (LCD) activity, and mRNA expression were increased by chilling stress but reduced by MPK inhibitor PD98059; PD98059 reversed the alleviating effect of H<sub>2</sub>S via increasing the EL and H<sub>2</sub>O<sub>2</sub> contents. The expression levels of <i>MPK1</i>-<i>MPK7</i> at low temperatures showed that <i>SlMPK4</i> was significantly induced by exogenous NaHS and showed a trend of first increasing and then decreasing, while the expression level of <i>SlMPK4</i> in HT-treated seedlings was lower than that of the control. After <i>SlMPK4</i> was silenced by virus-induced gene silencing, the H<sub>2</sub>S-induced upregulation of C-repeat-Binding Factor (CBF1), inducer of CBF expression 1 (ICE1), respiratory burst oxidase homologs (RBOH1, RBOH2) at low temperatures disappeared, and tomato cold tolerance decreased. In conclusion, H<sub>2</sub>S improves the cold tolerance of tomato plants by increasing the activity of antioxidant enzymes and reducing reactive oxygen species (ROS) accumulation and membrane lipid peroxidation. MPK4 may act as a downstream signaling molecule in this process."],"journal":["Antioxidants (Basel, Switzerland)"],"pubmed_title":["Hydrogen Sulfide Alleviates Oxidative Damage under Chilling Stress through Mitogen-Activated Protein Kinase in Tomato."],"pmcid":["PMC10967493"],"funding_grant_id":["HARS-22-07-S","232102111026"],"pubmed_authors":["Li Y","Li S","Chen J","He X","Wu C","Wang F","Wu G","Cui D","Niu X"],"additional_accession":[]},"is_claimable":false,"name":"Hydrogen Sulfide Alleviates Oxidative Damage under Chilling Stress through Mitogen-Activated Protein Kinase in Tomato.","description":"Tomato is the vegetable with the largest greenhouse area in China, and low temperature is one of the main factors affecting tomato growth, yield, and quality. Hydrogen sulfide (H<sub>2</sub>S) plays an important role in regulating plant chilling tolerance, but its downstream cascade reaction and mechanism remain unclear. Mitogen-activated protein kinases (MAPK/MPKs) are closely related to a variety of signaling substances in stress signal transmission. However, whether H<sub>2</sub>S is related to the MPK cascade pathway in response to low-temperature stress is rarely reported. In this study, NaHS treatment significantly decreased the electrolyte leakage (EL), superoxide anion (O<sub>2</sub><sup>-</sup>) production rate, and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) content of seedlings at low temperatures. In addition, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were obviously increased; and the photochemical efficiency of PSII (Fv/Fm) was enhanced with treatment with NaHS, indicating that NaHS improved the seedlings' cold tolerance by alleviating the degree of membrane lipid peroxidation and oxidative damage. However, H<sub>2</sub>S scavenger hypotaurine (HT) treatment showed the opposite effect. We found that H<sub>2</sub>S content, L-cysteine desulfhydrase (LCD) activity, and mRNA expression were increased by chilling stress but reduced by MPK inhibitor PD98059; PD98059 reversed the alleviating effect of H<sub>2</sub>S via increasing the EL and H<sub>2</sub>O<sub>2</sub> contents. The expression levels of <i>MPK1</i>-<i>MPK7</i> at low temperatures showed that <i>SlMPK4</i> was significantly induced by exogenous NaHS and showed a trend of first increasing and then decreasing, while the expression level of <i>SlMPK4</i> in HT-treated seedlings was lower than that of the control. After <i>SlMPK4</i> was silenced by virus-induced gene silencing, the H<sub>2</sub>S-induced upregulation of C-repeat-Binding Factor (CBF1), inducer of CBF expression 1 (ICE1), respiratory burst oxidase homologs (RBOH1, RBOH2) at low temperatures disappeared, and tomato cold tolerance decreased. In conclusion, H<sub>2</sub>S improves the cold tolerance of tomato plants by increasing the activity of antioxidant enzymes and reducing reactive oxygen species (ROS) accumulation and membrane lipid peroxidation. MPK4 may act as a downstream signaling molecule in this process.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-21T21:28:11.539Z","creation":"2025-04-05T18:24:57.634Z"},"accession":"S-EPMC10967493","cross_references":{"pubmed":["38539856"],"doi":["10.3390/antiox13030323"]}}