{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(5)"],"submitter":["Cao X"],"pubmed_abstract":["Muscle satellite cells (SCs) play a crucial role in the regeneration and repair of skeletal muscle injuries. Previous studies have shown that myogenic exosomes can enhance satellite cell proliferation, while the expression of miR-140-5p is significantly reduced during the repair process of mouse skeletal muscle injuries induced by BaCl2. This study aims to investigate the potential of myogenic exosomes carrying miR-140-5p inhibitors to activate SCs and influence the regeneration of injured muscles. Myogenic progenitor cell exosomes (MPC-Exo) and contained miR-140-5p mimics/inhibitors myogenic exosomes (MPC-Exo<sup>140+</sup> and MPC-Exo<sup>140-</sup>) were employed to treat SCs and use the model. The results demonstrate that miR-140-5p regulates SC proliferation by targeting <i>Pax7</i>. Upon the addition of MPC-Exo and MPC-Exo<sup>140-</sup>, <i>Pax7</i> expression in SCs significantly increased, leading to the transition of the cell cycle from G1 to S phase and an enhancement in cell proliferation. Furthermore, the therapeutic effect of MPC-Exo<sup>140-</sup> was validated in animal model, where the expression of muscle growth-related genes substantially increased in the gastrocnemius muscle. Our research demonstrates that MPC-Exo<sup>140-</sup> can effectively activate dormant muscle satellite cells, initiating their proliferation and differentiation processes, ultimately leading to the formation of new skeletal muscle cells and promoting skeletal muscle repair and remodeling."],"journal":["Aging"],"pagination":["4609-4630"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10968704"],"repository":["biostudies-literature"],"pubmed_title":["Myogenic exosome miR-140-5p modulates skeletal muscle regeneration and injury repair by regulating muscle satellite cells."],"pmcid":["PMC10968704"],"pubmed_authors":["Cao X","Lu J","Xue L","Luo X","Wang H","Yan Y","Wang J","Yu X"],"additional_accession":[]},"is_claimable":false,"name":"Myogenic exosome miR-140-5p modulates skeletal muscle regeneration and injury repair by regulating muscle satellite cells.","description":"Muscle satellite cells (SCs) play a crucial role in the regeneration and repair of skeletal muscle injuries. Previous studies have shown that myogenic exosomes can enhance satellite cell proliferation, while the expression of miR-140-5p is significantly reduced during the repair process of mouse skeletal muscle injuries induced by BaCl2. This study aims to investigate the potential of myogenic exosomes carrying miR-140-5p inhibitors to activate SCs and influence the regeneration of injured muscles. Myogenic progenitor cell exosomes (MPC-Exo) and contained miR-140-5p mimics/inhibitors myogenic exosomes (MPC-Exo<sup>140+</sup> and MPC-Exo<sup>140-</sup>) were employed to treat SCs and use the model. The results demonstrate that miR-140-5p regulates SC proliferation by targeting <i>Pax7</i>. Upon the addition of MPC-Exo and MPC-Exo<sup>140-</sup>, <i>Pax7</i> expression in SCs significantly increased, leading to the transition of the cell cycle from G1 to S phase and an enhancement in cell proliferation. Furthermore, the therapeutic effect of MPC-Exo<sup>140-</sup> was validated in animal model, where the expression of muscle growth-related genes substantially increased in the gastrocnemius muscle. Our research demonstrates that MPC-Exo<sup>140-</sup> can effectively activate dormant muscle satellite cells, initiating their proliferation and differentiation processes, ultimately leading to the formation of new skeletal muscle cells and promoting skeletal muscle repair and remodeling.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Feb","modification":"2025-04-04T23:52:53.942Z","creation":"2025-04-04T23:52:53.942Z"},"accession":"S-EPMC10968704","cross_references":{"pubmed":["38428405"],"doi":["10.18632/aging.205617"]}}