biostudies-literatureNguyen SLTVietnam National Foundation for Science and Technology Development1768-1776https://www.ebi.ac.uk/biostudies/studies/S-EPMC10969373biostudies-literatureUnknown46(3)Prodigiosin, a red pigment produced by numerous bacterial species, exerts various antibiotic effects on prokaryotic and eukaryotic organisms. For instance, human carcinoma cell lines appear to suffer from endoplasmic reticulum (ER) stress in the presence of prodigiosin. Here, we demonstrated that prodigiosin also triggers the unfolded-protein response (UPR), which is a cytoprotective response against ER stress, in yeast <i>Saccharomyces cerevisiae</i>. An <i>S. cerevisiae</i> mutant carrying a UPR-deficient mutation was hypersensitive to prodigiosin. Our observations cumulatively indicate that protein folding in the ER is impaired by prodigiosin, illustrating a new mode of action.Current issues in molecular biologyInduction of Endoplasmic Reticulum Stress by Prodigiosin in Yeast <i>Saccharomyces cerevisiae</i>.PMC10969373106.02-2018.332Nguyen TATNguyen TTDo TTLe THNguyen THTKimata YNguyen SLTfalseInduction of Endoplasmic Reticulum Stress by Prodigiosin in Yeast <i>Saccharomyces cerevisiae</i>.Prodigiosin, a red pigment produced by numerous bacterial species, exerts various antibiotic effects on prokaryotic and eukaryotic organisms. For instance, human carcinoma cell lines appear to suffer from endoplasmic reticulum (ER) stress in the presence of prodigiosin. Here, we demonstrated that prodigiosin also triggers the unfolded-protein response (UPR), which is a cytoprotective response against ER stress, in yeast <i>Saccharomyces cerevisiae</i>. An <i>S. cerevisiae</i> mutant carrying a UPR-deficient mutation was hypersensitive to prodigiosin. Our observations cumulatively indicate that protein folding in the ER is impaired by prodigiosin, illustrating a new mode of action.2024-01-01T00:00:00Z2024 Feb2025-04-04T23:52:59.248Z2025-04-04T23:52:59.248ZS-EPMC109693733853473210.3390/cimb46030116