{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nugnes M"],"funding":["Italian Ministry for Education, Universities and Research (MIUR) and University of Bologna (RFO funding scheme and Alma Idea 2017 Junior intramural grant)"],"pagination":["3168"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10970212"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(6)"],"pubmed_abstract":["Diabetic kidney disease (DKD) is a major cause of morbidity and mortality in individuals with type 2 diabetes mellitus (T2DM). The aim of this study was to investigate whether albumin structural alterations correlate with DKD severity and evaluate whether native and reduced albumin concentrations could complement the diagnosis of DKD. To this end, one hundred and seventeen T2DM patients without (<i>n</i> = 42) and with (<i>n</i> = 75) DKD (DKD I-III upon KDIGO classification) were evaluated; the total albumin concentration (tHA) was quantified by a bromocresol green assay, while structural alterations were profiled via liquid chromatography-high-resolution mass spectrometry (LC-HRMS). The concentrations of native albumin (eHA, effective albumin) and reduced albumin (rHA) were subsequently assessed. The HRMS analyses revealed a reduced relative amount of native albumin in DKD patients along with an increased abundance of altered forms, especially those bearing oxidative modifications. Accordingly, both eHA and rHA values varied during the stages of progressive renal failure, and these alterations were dose-dependently correlated with renal dysfunction. A ROC curve analysis revealed a significantly greater sensitivity and specificity of eHA and rHA than of tHA for diagnosing DKD. Importantly, according to the multivariate logistic regression analysis, the eHA was identified as an independent predictor of DKD."],"journal":["International journal of molecular sciences"],"pubmed_title":["Association between Albumin Alterations and Renal Function in Patients with Type 2 Diabetes Mellitus."],"pmcid":["PMC10970212"],"funding_grant_id":["Almaidea 2017"],"pubmed_authors":["Vetrano D","Marchignoli F","La Manna G","Petroni ML","Naldi M","Tedesco D","Capelli I","Pompili E","Ribichini D","Marchesini G","Bartolini M","Brodosi L","Nugnes M","Baldassarre M"],"additional_accession":[]},"is_claimable":false,"name":"Association between Albumin Alterations and Renal Function in Patients with Type 2 Diabetes Mellitus.","description":"Diabetic kidney disease (DKD) is a major cause of morbidity and mortality in individuals with type 2 diabetes mellitus (T2DM). The aim of this study was to investigate whether albumin structural alterations correlate with DKD severity and evaluate whether native and reduced albumin concentrations could complement the diagnosis of DKD. To this end, one hundred and seventeen T2DM patients without (<i>n</i> = 42) and with (<i>n</i> = 75) DKD (DKD I-III upon KDIGO classification) were evaluated; the total albumin concentration (tHA) was quantified by a bromocresol green assay, while structural alterations were profiled via liquid chromatography-high-resolution mass spectrometry (LC-HRMS). The concentrations of native albumin (eHA, effective albumin) and reduced albumin (rHA) were subsequently assessed. The HRMS analyses revealed a reduced relative amount of native albumin in DKD patients along with an increased abundance of altered forms, especially those bearing oxidative modifications. Accordingly, both eHA and rHA values varied during the stages of progressive renal failure, and these alterations were dose-dependently correlated with renal dysfunction. A ROC curve analysis revealed a significantly greater sensitivity and specificity of eHA and rHA than of tHA for diagnosing DKD. Importantly, according to the multivariate logistic regression analysis, the eHA was identified as an independent predictor of DKD.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-04T23:52:42.204Z","creation":"2025-04-04T23:52:42.204Z"},"accession":"S-EPMC10970212","cross_references":{"pubmed":["38542146"],"doi":["10.3390/ijms25063168"]}}