{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wu B"],"funding":["The Science & Technology Innovation Project of Laoshan Laboratory"],"pagination":["3413"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10970311"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(6)"],"pubmed_abstract":["Mesenchymal-epithelial transition (MET) is a widely spread and evolutionarily conserved process across species during development. In <i>Ciona</i> embryogenesis, the notochord cells undergo the transition from the non-polarized mesenchymal state into the polarized endothelial-like state to initiate the lumen formation between adjacent cells. Based on previously screened MET-related transcription factors by ATAC-seq and Smart-Seq of notochord cells, <i>Ciona robusta Snail</i> (<i>Ci-Snail</i>) was selected for its high-level expression during this period. Our current knockout results demonstrated that <i>Ci-Snail</i> was required for notochord cell MET. Importantly, overexpression of the transcription factor Brachyury in notochord cells resulted in a similar phenotype with failure of lumen formation and MET. More interestingly, expression of <i>Ci-Snail</i> in the notochord cells at the late tailbud stage could partially rescue the MET defect caused by Brachyury-overexpression. These results indicated an inverse relationship between <i>Ci-Snail</i> and <i>Brachyury</i> during notochord cell MET, which was verified by RT-qPCR analysis. Moreover, the overexpression of <i>Ci-Snail</i> could significantly inhibit the transcription of <i>Brachyury</i>, and the CUT&Tag-qPCR analysis demonstrated that <i>Ci-Snail</i> is directly bound to the upstream region of <i>Brachyury</i>. In summary, we revealed that <i>Ci-Snail</i> promoted the notochord cell MET and was essential for lumen formation via transcriptionally repressing <i>Brachyury</i>."],"journal":["International journal of molecular sciences"],"pubmed_title":["Snail Transcriptionally Represses <i>Brachyury</i> to Promote the Mesenchymal-Epithelial Transition in Ascidian Notochord Cells."],"pmcid":["PMC10970311"],"funding_grant_id":["No. LSKJ202203204"],"pubmed_authors":["Yang X","Ouyang X","Wu B","Dong B"],"additional_accession":[]},"is_claimable":false,"name":"Snail Transcriptionally Represses <i>Brachyury</i> to Promote the Mesenchymal-Epithelial Transition in Ascidian Notochord Cells.","description":"Mesenchymal-epithelial transition (MET) is a widely spread and evolutionarily conserved process across species during development. In <i>Ciona</i> embryogenesis, the notochord cells undergo the transition from the non-polarized mesenchymal state into the polarized endothelial-like state to initiate the lumen formation between adjacent cells. Based on previously screened MET-related transcription factors by ATAC-seq and Smart-Seq of notochord cells, <i>Ciona robusta Snail</i> (<i>Ci-Snail</i>) was selected for its high-level expression during this period. Our current knockout results demonstrated that <i>Ci-Snail</i> was required for notochord cell MET. Importantly, overexpression of the transcription factor Brachyury in notochord cells resulted in a similar phenotype with failure of lumen formation and MET. More interestingly, expression of <i>Ci-Snail</i> in the notochord cells at the late tailbud stage could partially rescue the MET defect caused by Brachyury-overexpression. These results indicated an inverse relationship between <i>Ci-Snail</i> and <i>Brachyury</i> during notochord cell MET, which was verified by RT-qPCR analysis. Moreover, the overexpression of <i>Ci-Snail</i> could significantly inhibit the transcription of <i>Brachyury</i>, and the CUT&Tag-qPCR analysis demonstrated that <i>Ci-Snail</i> is directly bound to the upstream region of <i>Brachyury</i>. In summary, we revealed that <i>Ci-Snail</i> promoted the notochord cell MET and was essential for lumen formation via transcriptionally repressing <i>Brachyury</i>.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2024-12-03T23:57:28.024Z","creation":"2024-12-03T23:57:28.024Z"},"accession":"S-EPMC10970311","cross_references":{"pubmed":["38542387"],"doi":["10.3390/ijms25063413"]}}