{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kang Y"],"funding":["Ministry of Education","Regional Innovation Strategy (RIS)\" through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (MOE)"],"pagination":["3175"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10970622"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(6)"],"pubmed_abstract":["Bifidobacteria are probiotic microorganisms commonly found in the gastrointestinal tract, some of which are known to utilize linear arabino-oligosaccharides (AOS) as prebiotic carbohydrates. In general, the synergistic actions of exo-type α-l-arabinofuranosidases (ABFs) and endo-α-1,5-l-arabinanases (ABNs) are required for efficient arabinan degradation. In this study, the putative gene cluster for arabinan degradation was discovered in the genome of <i>Bifidobacterium longum</i> subsp. <i>suis</i>. It consists of a variety of genes encoding exo- and endo-hydrolases, sugar-binding proteins, ABC-binding cassettes, and transcriptional regulators. Among them, two endo-ABNs GH43 (BflsABN43A and BflsABN43B), two exo-ABFs GH43 (BflsABF43A and BflsABF43B), and an exo-ABF GH51 (BflsABF51) were predicted to be the key hydrolases for arabinan degradation. These hydrolase genes were functionally expressed in <i>Escherichia coli</i>, and their enzymatic properties were characterized. Their synergism in arabinan degradation has been proposed from the detailed modes of action. Extracellular endo-BflsABN43A hydrolyzes sugar beet and debranched arabinans into the short-chain branched and linear AOS. Intracellularly, AOS can be further degraded into l-arabinose via the cooperative actions of endo-BflsABN43B, exo-BflsABF43A with debranching activity, α-1,5-linkage-specific exo-BflsABF43B, and exo-BflsABF51 with dual activities. The resulting l-arabinose is expected to be metabolized into energy through the pentose phosphate pathway by three enzymes expressed from the <i>ara</i> operon of bifidobacteria. It is anticipated that uncovering arabinan utilization gene clusters and their detailed functions in the genomes of diverse microorganisms will facilitate the development of customized synbiotics."],"journal":["International journal of molecular sciences"],"pubmed_title":["Functional Characterization of Endo- and Exo-Hydrolase Genes in Arabinan Degradation Gene Cluster of <i>Bifidobacterium longum</i> subsp. <i>suis</i>."],"pmcid":["PMC10970622"],"funding_grant_id":["NRF-2020R1F1A1076947","2021RIS-001 & NRF-2020R1F1A1076947","2021RIS-001"],"pubmed_authors":["Choi CY","Kim TJ","Ju YR","Han NS","Kang J","Kang Y","Kim HB"],"additional_accession":[]},"is_claimable":false,"name":"Functional Characterization of Endo- and Exo-Hydrolase Genes in Arabinan Degradation Gene Cluster of <i>Bifidobacterium longum</i> subsp. <i>suis</i>.","description":"Bifidobacteria are probiotic microorganisms commonly found in the gastrointestinal tract, some of which are known to utilize linear arabino-oligosaccharides (AOS) as prebiotic carbohydrates. In general, the synergistic actions of exo-type α-l-arabinofuranosidases (ABFs) and endo-α-1,5-l-arabinanases (ABNs) are required for efficient arabinan degradation. In this study, the putative gene cluster for arabinan degradation was discovered in the genome of <i>Bifidobacterium longum</i> subsp. <i>suis</i>. It consists of a variety of genes encoding exo- and endo-hydrolases, sugar-binding proteins, ABC-binding cassettes, and transcriptional regulators. Among them, two endo-ABNs GH43 (BflsABN43A and BflsABN43B), two exo-ABFs GH43 (BflsABF43A and BflsABF43B), and an exo-ABF GH51 (BflsABF51) were predicted to be the key hydrolases for arabinan degradation. These hydrolase genes were functionally expressed in <i>Escherichia coli</i>, and their enzymatic properties were characterized. Their synergism in arabinan degradation has been proposed from the detailed modes of action. Extracellular endo-BflsABN43A hydrolyzes sugar beet and debranched arabinans into the short-chain branched and linear AOS. Intracellularly, AOS can be further degraded into l-arabinose via the cooperative actions of endo-BflsABN43B, exo-BflsABF43A with debranching activity, α-1,5-linkage-specific exo-BflsABF43B, and exo-BflsABF51 with dual activities. The resulting l-arabinose is expected to be metabolized into energy through the pentose phosphate pathway by three enzymes expressed from the <i>ara</i> operon of bifidobacteria. It is anticipated that uncovering arabinan utilization gene clusters and their detailed functions in the genomes of diverse microorganisms will facilitate the development of customized synbiotics.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-21T21:27:16.595Z","creation":"2025-04-05T18:25:21.419Z"},"accession":"S-EPMC10970622","cross_references":{"pubmed":["38542148"],"doi":["10.3390/ijms25063175"]}}