biostudies-literatureUnknown12Lipovsek SMouse models of diet-induced type 2 diabetes mellitus provide powerful tools for studying the structural and physiological changes that are related to the disease progression. In this study, diabetic-like glucose dysregulation was induced in mice by feeding them a western diet, and light and transmission electron microscopy were used to study the ultrastructural changes in the pancreatic acinar cells. Acinar necrosis and vacuolization of the cytoplasm were the most prominent features. Furthermore, we observed intracellular and extracellular accumulation of lipid compounds in the form of lipid droplets, structural enlargement of the cisternae of the rough endoplasmic reticulum (RER), and altered mitochondrial morphology, with mitochondria lacking the typical organization of the inner membraFrontiers in cell and developmental biology1380564https://www.ebi.ac.uk/biostudies/studies/S-EPMC10972872biostudies-literatureWestern diet-induced ultrastructural changes in mouse pancreatic acinar cells.PMC10972872Vajs TDolensek JLipovsek SDaris BLeitinger GValladolid-Acebes ISkelin Klemen MStozer AfalseWestern diet-induced ultrastructural changes in mouse pancreatic acinar cells.Mouse models of diet-induced type 2 diabetes mellitus provide powerful tools for studying the structural and physiological changes that are related to the disease progression. In this study, diabetic-like glucose dysregulation was induced in mice by feeding them a western diet, and light and transmission electron microscopy were used to study the ultrastructural changes in the pancreatic acinar cells. Acinar necrosis and vacuolization of the cytoplasm were the most prominent features. Furthermore, we observed intracellular and extracellular accumulation of lipid compounds in the form of lipid droplets, structural enlargement of the cisternae of the rough endoplasmic reticulum (RER), and altered mitochondrial morphology, with mitochondria lacking the typical organization of the inner membra2024-01-01T00:00:00Z20242025-04-26T11:21:35.575Z2025-04-06T13:39:25.03ZS-EPMC109728723855037910.3389/fcell.2024.1380564