{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Glackin CC"],"funding":["BiodivERsA","Federal Ministry of Education and Research"],"pagination":["614"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10974389"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(3)"],"pubmed_abstract":["With rising infection rates in recent years, <i>Vibrio vulnificus</i> poses an increasing threat to public safety in the coastal brackish Baltic Sea. It is therefore important to monitor this organism and assess the <i>V. vulnificus</i> infection risk on a more regular basis. However, as the coastline of the Baltic Sea is 8000 km long and shared by nine nations, a convenient, fast, inexpensive, yet efficient <i>V. vulnificus</i> identification method is essential. We evaluated the effectiveness of a two-step agar-based approach consisting of successive <i>Vibrio</i> isolation and cultivation on thiosulphate-citrate-bile salt sucrose (TCBS) agar and CHROMagar™ <i>Vibrio</i> for <i>V. vulnificus</i> in comparison with <i>V. cholerae</i>, <i>V. parahaemolyticus</i>, and <i>V. alginolyticus</i>. Our study contains isolates from water and sediment across a broad expanse of the Baltic Sea including 13 locations and two different summers, the time of year during which <i>Vibrio</i> infections are usually much more frequent. Confirmation of isolate species identity was carried out using molecular analyses. The two-step agar plating method performed well across different locations and timeframes in correctly identifying <i>V. vulnificus</i> by more than 80%, but the sensitivity in other <i>Vibrio</i> species varied. Thus, our approach yielded promising results as a potential tool for early <i>V. vulnificus</i> detection across a broad timeframe and transect of the Baltic Sea and potentially other brackish environments."],"journal":["Microorganisms"],"pubmed_title":["Combined TCBS and CHROMagar Analyses Allow for Basic Identification of <i>Vibrio vulnificus</i> within a 48 h Incubation Period in the Coastal Baltic Sea."],"pmcid":["PMC10974389"],"funding_grant_id":["03ZU1107KA","16LC2022A"],"pubmed_authors":["Chandra TS","Dupke S","Labrenz M","Riedinger D","Glackin CC"],"additional_accession":[]},"is_claimable":false,"name":"Combined TCBS and CHROMagar Analyses Allow for Basic Identification of <i>Vibrio vulnificus</i> within a 48 h Incubation Period in the Coastal Baltic Sea.","description":"With rising infection rates in recent years, <i>Vibrio vulnificus</i> poses an increasing threat to public safety in the coastal brackish Baltic Sea. It is therefore important to monitor this organism and assess the <i>V. vulnificus</i> infection risk on a more regular basis. However, as the coastline of the Baltic Sea is 8000 km long and shared by nine nations, a convenient, fast, inexpensive, yet efficient <i>V. vulnificus</i> identification method is essential. We evaluated the effectiveness of a two-step agar-based approach consisting of successive <i>Vibrio</i> isolation and cultivation on thiosulphate-citrate-bile salt sucrose (TCBS) agar and CHROMagar™ <i>Vibrio</i> for <i>V. vulnificus</i> in comparison with <i>V. cholerae</i>, <i>V. parahaemolyticus</i>, and <i>V. alginolyticus</i>. Our study contains isolates from water and sediment across a broad expanse of the Baltic Sea including 13 locations and two different summers, the time of year during which <i>Vibrio</i> infections are usually much more frequent. Confirmation of isolate species identity was carried out using molecular analyses. The two-step agar plating method performed well across different locations and timeframes in correctly identifying <i>V. vulnificus</i> by more than 80%, but the sensitivity in other <i>Vibrio</i> species varied. Thus, our approach yielded promising results as a potential tool for early <i>V. vulnificus</i> detection across a broad timeframe and transect of the Baltic Sea and potentially other brackish environments.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-04-08T18:53:54.815Z","creation":"2025-04-04T19:11:32.75Z"},"accession":"S-EPMC10974389","cross_references":{"pubmed":["38543665"],"doi":["10.3390/microorganisms12030614"]}}