{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang C"],"funding":["Fundamental Research Funds for the Central Universities","National Natural Science Foundation of China"],"pagination":["10255-10261"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10975311"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(15)"],"pubmed_abstract":["Fluorescence imaging of organelles at the cellular level is important for studying biological processes. The development of a highly emissive fluorescent probe that operates under a suitable excitation light source is a key step in high-quality fluorescence imaging. For long-term, high-fidelity fluorescence imaging of mitochondria-related cellular processes using two-photon microscopy and stimulated emission depletion microscopy, we developed a new benzocoumarin-based cationic fluorescent probe (BS-CN) that is far-red emitting, water-soluble, photostable, and very bright in cells. BS-CN showed a remarkably high quantum yield of 0.35 and a large two-photon excited fluorescence action cross-section of 76 GM, enabling the long-term tracking of mitochondria in live cells. In addition, BS-CN exhibited a certain affinity for RNA and stained nucleoli in fixed cells. A comparative assessment of the photophysical properties and bioimaging performance of benzo[<i>h</i>]coumarin-pyridinium and the structurally similar styryl-pyridinium (BS-MN) clearly indicated the importance of structural rigidity for fluorescence efficiency."],"journal":["RSC advances"],"pubmed_title":["Rigidify styryl-pyridinium dyes to benzo[<i>h</i>]coumarin-based bright two-photon fluorescent probes for cellular bioimaging."],"pmcid":["PMC10975311"],"funding_grant_id":["U2330106","2022JC003","52350002"],"pubmed_authors":["Liu Y","Jin W","Liu Z","Zhang C","Yu Z","Yu X"],"additional_accession":[]},"is_claimable":false,"name":"Rigidify styryl-pyridinium dyes to benzo[<i>h</i>]coumarin-based bright two-photon fluorescent probes for cellular bioimaging.","description":"Fluorescence imaging of organelles at the cellular level is important for studying biological processes. The development of a highly emissive fluorescent probe that operates under a suitable excitation light source is a key step in high-quality fluorescence imaging. For long-term, high-fidelity fluorescence imaging of mitochondria-related cellular processes using two-photon microscopy and stimulated emission depletion microscopy, we developed a new benzocoumarin-based cationic fluorescent probe (BS-CN) that is far-red emitting, water-soluble, photostable, and very bright in cells. BS-CN showed a remarkably high quantum yield of 0.35 and a large two-photon excited fluorescence action cross-section of 76 GM, enabling the long-term tracking of mitochondria in live cells. In addition, BS-CN exhibited a certain affinity for RNA and stained nucleoli in fixed cells. A comparative assessment of the photophysical properties and bioimaging performance of benzo[<i>h</i>]coumarin-pyridinium and the structurally similar styryl-pyridinium (BS-MN) clearly indicated the importance of structural rigidity for fluorescence efficiency.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-26T11:24:07.571Z","creation":"2025-04-06T13:42:00.933Z"},"accession":"S-EPMC10975311","cross_references":{"pubmed":["38549794"],"doi":["10.1039/d3ra08269e"]}}