{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bai Y"],"funding":["Guangdong Provincial Key R&amp;amp;D Program","Heyuan Branch, Guangdong Laboratory for Lingnan Modern Agriculture Project","China Agriculture Research System of MOF and MARA","Guangdong Provincial Key R&D Program","Guangdong Basic and Applied Basic Research Foundation","Science and Technology Program of Guangdong province, China","Provincial Science and Technology Special Fund Project for Zhongshan City(major special project + Task list management mode)","Special Project of National Modern Agricultural Industrial Technology System","construction project of modern agricultural science and technology innovation alliance in Guangdong province","Provincial Science and Technology Special Fund Project for Zhongshan City (major special project + task list management mode)","Natural Science Foundation of Guangzhou"],"pagination":["122"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10975772"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(3)"],"pubmed_abstract":["Among broilers, the main pathogen that leads to swollen head syndrome (SHS) is the subgroup C avian metapneumovirus (aMPV-C). The aMPV-C infection can lead to an upsurge in the rate of soft-shell eggs, resulting in reduced egg production and seriously affecting the economy of the livestock industry. Therefore, a rapid method for aMPV-C detection needs to be invented. According to the N gene of aMPV-C, we designed the specific probe and primer and created a reverse transcription recombinase-aided amplification assay (RT-RAA) for the detection of aMPV-C. aMPV-C could be detected quickly and specifically by this method at 41 °C for 30 min. The sensitivity assay inferred that the minimum detection threshold of RT-RAA was 3.38 × 10<sup>1</sup> copies/μL. A specificity assay showed that the RT-RAA method did not cross-react with other subgroups (aMPV-A, aMPV-B, aMPV-D) or other viruses (H9N2, NDV, IBV, IBDV). Forty samples of known clinical background were tested by RT-RAA and RT-qPCR. The two approaches had a 100% correlation rate. In conclusion, this research successfully created an RT-RAA assay for aMPV-C."],"journal":["Veterinary sciences"],"pubmed_title":["Establishment and Application Prospect of Reverse Transcriptase Recombinase-Aided Amplification Assay for Subgroup C Avian Metapneumovirus."],"pmcid":["PMC10975772"],"funding_grant_id":["2023A1515010584","2021sdr003","2020KJ128","2020B020222001, 2019B020218004","2023A04J1461","2020B1212060060","2020B020222001","2021A1515010817","2023A1515010584, 2021A1515010817","DT20220003","2021KJ128","2019B020218004","2021KJ128, 2020KJ128","CARS-41","CARS-42-13"],"pubmed_authors":["Li H","Liu J","Li T","Zhang X","Dong M","Bai Y","Xie Q","Wang Z","Dai Z","Wu X"],"additional_accession":[]},"is_claimable":false,"name":"Establishment and Application Prospect of Reverse Transcriptase Recombinase-Aided Amplification Assay for Subgroup C Avian Metapneumovirus.","description":"Among broilers, the main pathogen that leads to swollen head syndrome (SHS) is the subgroup C avian metapneumovirus (aMPV-C). The aMPV-C infection can lead to an upsurge in the rate of soft-shell eggs, resulting in reduced egg production and seriously affecting the economy of the livestock industry. Therefore, a rapid method for aMPV-C detection needs to be invented. According to the N gene of aMPV-C, we designed the specific probe and primer and created a reverse transcription recombinase-aided amplification assay (RT-RAA) for the detection of aMPV-C. aMPV-C could be detected quickly and specifically by this method at 41 °C for 30 min. The sensitivity assay inferred that the minimum detection threshold of RT-RAA was 3.38 × 10<sup>1</sup> copies/μL. A specificity assay showed that the RT-RAA method did not cross-react with other subgroups (aMPV-A, aMPV-B, aMPV-D) or other viruses (H9N2, NDV, IBV, IBDV). Forty samples of known clinical background were tested by RT-RAA and RT-qPCR. The two approaches had a 100% correlation rate. In conclusion, this research successfully created an RT-RAA assay for aMPV-C.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-04-08T15:10:48.867Z","creation":"2025-04-05T23:16:18.352Z"},"accession":"S-EPMC10975772","cross_references":{"pubmed":["38535856"],"doi":["10.3390/vetsci11030122"]}}