biostudies-literatureBai YGuangdong Provincial Key R&D ProgramHeyuan Branch, Guangdong Laboratory for Lingnan Modern Agriculture ProjectChina Agriculture Research System of MOF and MARAGuangdong Provincial Key R&D ProgramGuangdong Basic and Applied Basic Research FoundationScience and Technology Program of Guangdong province, ChinaProvincial Science and Technology Special Fund Project for Zhongshan City(major special project + Task list management mode)Special Project of National Modern Agricultural Industrial Technology Systemconstruction project of modern agricultural science and technology innovation alliance in Guangdong provinceProvincial Science and Technology Special Fund Project for Zhongshan City (major special project + task list management mode)Natural Science Foundation of Guangzhou122https://www.ebi.ac.uk/biostudies/studies/S-EPMC10975772biostudies-literatureUnknown11(3)Among broilers, the main pathogen that leads to swollen head syndrome (SHS) is the subgroup C avian metapneumovirus (aMPV-C). The aMPV-C infection can lead to an upsurge in the rate of soft-shell eggs, resulting in reduced egg production and seriously affecting the economy of the livestock industry. Therefore, a rapid method for aMPV-C detection needs to be invented. According to the N gene of aMPV-C, we designed the specific probe and primer and created a reverse transcription recombinase-aided amplification assay (RT-RAA) for the detection of aMPV-C. aMPV-C could be detected quickly and specifically by this method at 41 °C for 30 min. The sensitivity assay inferred that the minimum detection threshold of RT-RAA was 3.38 × 10¹ copies/μL. A specificity assay showed that the RT-RAA method did not cross-react with other subgroups (aMPV-A, aMPV-B, aMPV-D) or other viruses (H9N2, NDV, IBV, IBDV). Forty samples of known clinical background were tested by RT-RAA and RT-qPCR. The two approaches had a 100% correlation rate. In conclusion, this research successfully created an RT-RAA assay for aMPV-C.Veterinary sciencesEstablishment and Application Prospect of Reverse Transcriptase Recombinase-Aided Amplification Assay for Subgroup C Avian Metapneumovirus.PMC109757722023A15150105842021sdr0032020KJ1282020B020222001, 2019B0202180042023A04J14612020B12120600602020B0202220012021A15150108172023A1515010584, 2021A1515010817DT202200032021KJ1282019B0202180042021KJ128, 2020KJ128CARS-41CARS-42-13Li HLiu JLi TZhang XDong MBai YXie QWang ZDai ZWu XfalseEstablishment and Application Prospect of Reverse Transcriptase Recombinase-Aided Amplification Assay for Subgroup C Avian Metapneumovirus.Among broilers, the main pathogen that leads to swollen head syndrome (SHS) is the subgroup C avian metapneumovirus (aMPV-C). The aMPV-C infection can lead to an upsurge in the rate of soft-shell eggs, resulting in reduced egg production and seriously affecting the economy of the livestock industry. Therefore, a rapid method for aMPV-C detection needs to be invented. According to the N gene of aMPV-C, we designed the specific probe and primer and created a reverse transcription recombinase-aided amplification assay (RT-RAA) for the detection of aMPV-C. aMPV-C could be detected quickly and specifically by this method at 41 °C for 30 min. The sensitivity assay inferred that the minimum detection threshold of RT-RAA was 3.38 × 10¹ copies/μL. A specificity assay showed that the RT-RAA method did not cross-react with other subgroups (aMPV-A, aMPV-B, aMPV-D) or other viruses (H9N2, NDV, IBV, IBDV). Forty samples of known clinical background were tested by RT-RAA and RT-qPCR. The two approaches had a 100% correlation rate. In conclusion, this research successfully created an RT-RAA assay for aMPV-C.2024-01-01T00:00:00Z2024 Mar2026-04-08T15:10:48.867Z2025-04-05T23:16:18.352ZS-EPMC109757723853585610.3390/vetsci11030122