biostudies-literatureMa XChina Scholarship Council1304325https://www.ebi.ac.uk/biostudies/studies/S-EPMC10977602biostudies-literatureUnknown15Microbial population heterogeneity leads to different stress responses and growth behavior of individual cells in a population. Previously, a point mutation in the <i>rpsU</i> gene (<i>rpsU</i>^G50C) encoding ribosomal protein S21 was identified in a <i>Listeria monocytogenes</i> LO28 variant, which leads to increased multi-stress resistance and a reduced maximum specific growth rate. However, the underlying mechanisms of these phenotypic changes remain unknown. In <i>L. monocytogenes</i>, the alternative sigma factor SigB regulates the general stress response, with its activation controlled by a series of Rsb proteins, including RsbR1 and anti-sigma factor RsbW and its antagonist RsbV. We combined a phenotype and proteomics approach to investigate the acid and heat stress resistFrontiers in microbiologyA single point mutation in the <i>Listeria monocytogenes</i> ribosomal gene <i>rpsU</i> enables SigB activation independently of the stressosome and the anti-sigma factor antagonist RsbV.PMC10977602201907720086Zwietering MHBoeren SMa XO'Byrne CPAbee TTempelaars MHden Besten HMWfalseA single point mutation in the <i>Listeria monocytogenes</i> ribosomal gene <i>rpsU</i> enables SigB activation independently of the stressosome and the anti-sigma factor antagonist RsbV.Microbial population heterogeneity leads to different stress responses and growth behavior of individual cells in a population. Previously, a point mutation in the <i>rpsU</i> gene (<i>rpsU</i>^G50C) encoding ribosomal protein S21 was identified in a <i>Listeria monocytogenes</i> LO28 variant, which leads to increased multi-stress resistance and a reduced maximum specific growth rate. However, the underlying mechanisms of these phenotypic changes remain unknown. In <i>L. monocytogenes</i>, the alternative sigma factor SigB regulates the general stress response, with its activation controlled by a series of Rsb proteins, including RsbR1 and anti-sigma factor RsbW and its antagonist RsbV. We combined a phenotype and proteomics approach to investigate the acid and heat stress resist2024-01-01T00:00:00Z20242026-05-29T10:05:07.937Z2024-11-20T08:41:17.818ZS-EPMC109776023855086510.3389/fmicb.2024.1304325