biostudies-literatureUnknown11(4)van Henten SInstitute of Tropical MedicineDirectorate-General for Development Cooperation and Humanitarian AidUniversity of Gondar<h4>Background</h4>Diagnosis of cutaneous leishmaniasis (CL) usually relies on invasive samples, but it is unclear whether more patient-friendly tools are good alternatives for diverse lesions when used with polymerase chain reaction (PCR).<h4>Methods</h4>Patients with suspected CL were enrolled consecutively in a prospective diagnostic accuracy study. We compared dental broach, tape disc, and microbiopsy samples with PCR as index tests, using PCR with skin slit samples as reference test. Subsequently, we constructed a composite reference test including microscopy, the 3 index tests and skin slit PCR, and we compared these same tests with the composite reference test. We assessed diagnostic accuracy parameters with 95% confidence intervals for all comparisons.<h4>Results</h4>Among 344 inclOpen forum infectious diseasesofae113https://www.ebi.ac.uk/biostudies/studies/S-EPMC10977625biostudies-literatureEvaluation of Less Invasive Sampling Tools for the Diagnosis of Cutaneous Leishmaniasis.PMC10977625Engidaw AVogt FMekonnen TMoons KGMMulaw TMelkamu RDessie Dvan Henten SPareyn Mvan Griensven JCnops LFikre HBogale TYeshanew AKassa MfalseEvaluation of Less Invasive Sampling Tools for the Diagnosis of Cutaneous Leishmaniasis.<h4>Background</h4>Diagnosis of cutaneous leishmaniasis (CL) usually relies on invasive samples, but it is unclear whether more patient-friendly tools are good alternatives for diverse lesions when used with polymerase chain reaction (PCR).<h4>Methods</h4>Patients with suspected CL were enrolled consecutively in a prospective diagnostic accuracy study. We compared dental broach, tape disc, and microbiopsy samples with PCR as index tests, using PCR with skin slit samples as reference test. Subsequently, we constructed a composite reference test including microscopy, the 3 index tests and skin slit PCR, and we compared these same tests with the composite reference test. We assessed diagnostic accuracy parameters with 95% confidence intervals for all comparisons.<h4>Results</h4>Among 344 incl2024-01-01T00:00:00Z2024 Apr2026-04-08T15:17:55.976Z2025-04-06T07:44:49.852ZS-EPMC109776253856060010.1093/ofid/ofae113