{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nagy GN"],"funding":["Carlsbergfondet","Danmarks Grundforskningsfond","Medical Research Council","NIMH NIH HHS","Dr Miriam and Sheldon G. Adelson Medical Foundation","Wellcome Trust","Novo Nordisk Fonden","U.S. Department of Health & Human Services | National Institutes of Health"],"pagination":["2723"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10978931"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["Integration of extracellular signals by neurons is pivotal for brain development, plasticity, and repair. Axon guidance relies on receptor-ligand interactions crosstalking with extracellular matrix components. Semaphorin-5A (Sema5A) is a bifunctional guidance cue exerting attractive and inhibitory effects on neuronal growth through the interaction with heparan sulfate (HS) and chondroitin sulfate (CS) glycosaminoglycans (GAGs), respectively. Sema5A harbors seven thrombospondin type-1 repeats (TSR1-7) important for GAG binding, however the underlying molecular basis and functions in vivo remain enigmatic. Here we dissect the structural basis for Sema5A:GAG specificity and demonstrate the functional significance of this interaction in vivo. Using x-ray crystallography, we reveal a dimeric fo"],"journal":["Nature communications"],"pubmed_title":["Structure and function of Semaphorin-5A glycosaminoglycan interactions."],"pmcid":["PMC10978931"],"funding_grant_id":["203141/Z/16/Z","NNF22OC0073736","MR/T000503/1","223133/Z/21/Z","CF20-0412","R01 MH119346","DNRF107"],"pubmed_authors":["Zhao XF","Miller RL","El Omari K","Duman R","Clausen H","Wagner A","Nagy GN","Harlos K","Karlsson R","Wang K","Giger RJ","Jones EY"],"additional_accession":[]},"is_claimable":false,"name":"Structure and function of Semaphorin-5A glycosaminoglycan interactions.","description":"Integration of extracellular signals by neurons is pivotal for brain development, plasticity, and repair. Axon guidance relies on receptor-ligand interactions crosstalking with extracellular matrix components. Semaphorin-5A (Sema5A) is a bifunctional guidance cue exerting attractive and inhibitory effects on neuronal growth through the interaction with heparan sulfate (HS) and chondroitin sulfate (CS) glycosaminoglycans (GAGs), respectively. Sema5A harbors seven thrombospondin type-1 repeats (TSR1-7) important for GAG binding, however the underlying molecular basis and functions in vivo remain enigmatic. Here we dissect the structural basis for Sema5A:GAG specificity and demonstrate the functional significance of this interaction in vivo. Using x-ray crystallography, we reveal a dimeric fo","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-06-01T21:47:32.399Z","creation":"2025-04-04T19:12:32.806Z"},"accession":"S-EPMC10978931","cross_references":{"pubmed":["38548715"],"doi":["10.1038/s41467-024-46725-7"]}}