{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["28(3)"],"submitter":["Huang P"],"pubmed_abstract":["<b><i>Background:</i></b> Ferroptosis is associated with tumor development; however, its contribution to radioresistant head and neck cancer (HNC) remains unclear. In this study, we used bioinformatics analysis and <i>in vitro</i> testing to explore ferroptosis-related genes associated with HNCs radiosensitivity. <b><i>Materials and Methods:</i></b> GSE9714, GSE90761, and The Cancer Genome Atlas (TCGA) datasets were searched to identify ferroptosis-related differentially expressed genes between radioresistant and radiosensitive HNCs or radiation-treated and nonradiation-treated HNCs. A protein-protein interaction analysis on identified hub genes was then performed. Receiver operating characteristic curves and Kaplan-Meier survival analysis were used to assess the diagnostic and prognostic potential of the hub genes. Cell counting kit-8, transwell assay, and flow cytometry were applied to examine the role of hub gene collagen type IV, alpha1 chain (<i>COL4A1</i>) on the proliferation, migration, invasion, and apoptosis of TU686 cells. <b><i>Results:</i></b> Hub genes <i>MMP10, MMP1, COL4A1, IFI27</i>, and <i>INHBA</i> showed diagnostic potential for HNC and were negatively correlated with overall survival and disease-free survival in the TCGA dataset. Also, <i>IL-1B, IFI27, INHBA,</i> and <i>COL4A1</i> mRNA levels were significantly increased in TCGA patients with advanced clinical stages or receiving radiotherapy, whereas <i>COL4A1, MMP10</i>, and <i>INHBA</i> expressions were negatively correlated with immune infiltration. Furthermore, the knockdown of <i>COL4A1</i> inhibited cell proliferation, migration, and invasion while promoting apoptosis in TU686 cells. <b><i>Conclusion:</i></b> Ferroptosis-related hub genes, such as <i>COL4A1,</i> are potential diagnostic and prognostic indicators as well as therapeutic targets for HNC."],"journal":["Genetic testing and molecular biomarkers"],"pagination":["100-113"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10979683"],"repository":["biostudies-literature"],"pubmed_title":["Ferroptosis-Related Genes Are Associated with Radioresistance and Immune Suppression in Head and Neck Cancer."],"pmcid":["PMC10979683"],"pubmed_authors":["Wang R","Ning X","Kang M","Huang P"],"additional_accession":[]},"is_claimable":false,"name":"Ferroptosis-Related Genes Are Associated with Radioresistance and Immune Suppression in Head and Neck Cancer.","description":"<b><i>Background:</i></b> Ferroptosis is associated with tumor development; however, its contribution to radioresistant head and neck cancer (HNC) remains unclear. In this study, we used bioinformatics analysis and <i>in vitro</i> testing to explore ferroptosis-related genes associated with HNCs radiosensitivity. <b><i>Materials and Methods:</i></b> GSE9714, GSE90761, and The Cancer Genome Atlas (TCGA) datasets were searched to identify ferroptosis-related differentially expressed genes between radioresistant and radiosensitive HNCs or radiation-treated and nonradiation-treated HNCs. A protein-protein interaction analysis on identified hub genes was then performed. Receiver operating characteristic curves and Kaplan-Meier survival analysis were used to assess the diagnostic and prognostic potential of the hub genes. Cell counting kit-8, transwell assay, and flow cytometry were applied to examine the role of hub gene collagen type IV, alpha1 chain (<i>COL4A1</i>) on the proliferation, migration, invasion, and apoptosis of TU686 cells. <b><i>Results:</i></b> Hub genes <i>MMP10, MMP1, COL4A1, IFI27</i>, and <i>INHBA</i> showed diagnostic potential for HNC and were negatively correlated with overall survival and disease-free survival in the TCGA dataset. Also, <i>IL-1B, IFI27, INHBA,</i> and <i>COL4A1</i> mRNA levels were significantly increased in TCGA patients with advanced clinical stages or receiving radiotherapy, whereas <i>COL4A1, MMP10</i>, and <i>INHBA</i> expressions were negatively correlated with immune infiltration. Furthermore, the knockdown of <i>COL4A1</i> inhibited cell proliferation, migration, and invasion while promoting apoptosis in TU686 cells. <b><i>Conclusion:</i></b> Ferroptosis-related hub genes, such as <i>COL4A1,</i> are potential diagnostic and prognostic indicators as well as therapeutic targets for HNC.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-06-01T21:47:23.759Z","creation":"2025-04-04T19:12:24.323Z"},"accession":"S-EPMC10979683","cross_references":{"pubmed":["38478802"],"doi":["10.1089/gtmb.2023.0193"]}}