biostudies-literatureLegeai FFrench National Research Agencyevae055https://www.ebi.ac.uk/biostudies/studies/S-EPMC10980516biostudies-literatureUnknown16(3)We present the first chromosome-level genome assembly and annotation of the pearly heath Coenonympha arcania, generated with a PacBio HiFi sequencing approach and complemented with Hi-C data. We additionally compare synteny, gene, and repeat content between C. arcania and other Lepidopteran genomes. This reference genome will enable future population genomics studies with Coenonympha butterflies, a species-rich genus that encompasses some of the most highly endangered butterfly taxa in Europe.Genome biology and evolutionChromosome-Level Assembly and Annotation of the Pearly Heath Coenonympha arcania Butterfly Genome.PMC10980516ANR-20-CE02-0017Lemaitre CRomain SDoniol-Valcroze PJoron MLegeai FDespres LCapblancq TfalseChromosome-Level Assembly and Annotation of the Pearly Heath Coenonympha arcania Butterfly Genome.We present the first chromosome-level genome assembly and annotation of the pearly heath Coenonympha arcania, generated with a PacBio HiFi sequencing approach and complemented with Hi-C data. We additionally compare synteny, gene, and repeat content between C. arcania and other Lepidopteran genomes. This reference genome will enable future population genomics studies with Coenonympha butterflies, a species-rich genus that encompasses some of the most highly endangered butterfly taxa in Europe.2024-01-01T00:00:00Z2024 Mar2026-06-01T21:47:19.325Z2025-04-04T19:12:20.108ZS-EPMC109805163849196910.1093/gbe/evae055