biostudies-literatureNieto-Rosado MBill and Melinda Gates Foundation (Bill & Melinda Gates Foundation)Bill and Melinda Gates Foundation2758https://www.ebi.ac.uk/biostudies/studies/S-EPMC10980694biostudies-literatureUnknown15(1)Hospital surfaces can harbour bacterial pathogens, which may disseminate and cause nosocomial infections, contributing towards mortality in low- and middle-income countries (LMICs). During the BARNARDS study, hospital surfaces from neonatal wards were sampled to assess the degree of environmental surface and patient care equipment colonisation by Gram-negative bacteria (GNB) carrying antibiotic resistance genes (ARGs). Here, we perform PCR screening for extended-spectrum β-lactamases (bla_CTX-M-15) and carbapenemases (bla_NDM, bla_OXA-48-like and bla_KPC), MALDI-TOF MS identification of GNB carrying ARGs, and further analysis by whole genome sequencing of bacterial isolates. We determine presence of consistently dominant clones and their relatedness Nature communicationsColonisation of hospital surfaces from low- and middle-income countries by extended spectrum β-lactamase- and carbapenemase-producing bacteria.PMC10980694OP1191522OPP1119772Boostrom IGaju LIregbu KCZahra RUllah SNModibbo FMukkadas ASBulabula ANHSands KAkpulu CSanches Ferreira ADMazarati JBNieto-Rosado MAdeleye AAndrews RSaif HAkif SAudu LEdwin CPPortal EARMilton RYusuf AHBekele DChan GAbayneh MPortal BDyer CMuhammad AMetaferia GShirazi HGroup BARNARDSCarvalho MJWatkins WJMehtar SWhitelaw ASolomon SRucogoza AJan MHWalsh TRThomson KMMathias JRoberts LHender THogan PUwaezuoke SfalseColonisation of hospital surfaces from low- and middle-income countries by extended spectrum β-lactamase- and carbapenemase-producing bacteria.Hospital surfaces can harbour bacterial pathogens, which may disseminate and cause nosocomial infections, contributing towards mortality in low- and middle-income countries (LMICs). During the BARNARDS study, hospital surfaces from neonatal wards were sampled to assess the degree of environmental surface and patient care equipment colonisation by Gram-negative bacteria (GNB) carrying antibiotic resistance genes (ARGs). Here, we perform PCR screening for extended-spectrum β-lactamases (bla_CTX-M-15) and carbapenemases (bla_NDM, bla_OXA-48-like and bla_KPC), MALDI-TOF MS identification of GNB carrying ARGs, and further analysis by whole genome sequencing of bacterial isolates. We determine presence of consistently dominant clones and their relatedness 2024-01-01T00:00:00Z2024 Mar2025-04-04T20:16:34.312Z2025-04-04T20:16:34.312ZS-EPMC109806943855343910.1038/s41467-024-46684-z