{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(3)"],"submitter":["Zhang B"],"pubmed_abstract":["Leaf color is an important agronomic trait in cabbage (<i>Brassica oleracea</i> L. var. <i>capitata</i>), but the detailed mechanism underlying leaf color formation remains unclear. In this study, we characterized a <i>Brassica oleracea yellow-green leaf 2</i> (<i>BoYgl-2</i>) mutant 4036Y, which has significantly reduced chlorophyll content and abnormal chloroplasts during early leaf development. Genetic analysis revealed that the yellow-green leaf trait is controlled by a single recessive gene. Map-based cloning revealed that <i>BoYgl-2</i> encodes a novel nuclear-targeted P-type PPR protein, which is absent in the 4036Y mutant. Functional complementation showed that <i>BoYgl-2</i> from the normal-green leaf 4036G can rescue the yellow-green leaf phenotype of 4036Y. The C-to-U editing efficiency and expression levels of <i>atpF</i>, <i>rps14</i>, <i>petL</i> and <i>ndhD</i> were significantly reduced in 4036Y than that in 4036G, and significantly increased in <i>BoYgl-2</i> overexpression lines than that in 4036Y. The expression levels of many plastid- and nuclear-encoded genes associated with chloroplast development in <i>BoYgl-2</i> mutant were also significantly altered. These results suggest that <i>BoYgl-2</i> participates in chloroplast C-to-U editing and development, which provides rare insight into the molecular mechanism underlying leaf color formation in cabbage."],"journal":["Horticulture research"],"pagination":["uhae006"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10980974"],"repository":["biostudies-literature"],"pubmed_title":["Chloroplast C-to-U editing, regulated by a PPR protein BoYgl-2, is important for chlorophyll biosynthesis in cabbage."],"pmcid":["PMC10980974"],"pubmed_authors":["Ren W","Zhuang M","Li S","Yang L","Lv H","Zhang Y","Ji J","Wang Y","Hou X","Zhang B","Wu Y"],"additional_accession":[]},"is_claimable":false,"name":"Chloroplast C-to-U editing, regulated by a PPR protein BoYgl-2, is important for chlorophyll biosynthesis in cabbage.","description":"Leaf color is an important agronomic trait in cabbage (<i>Brassica oleracea</i> L. var. <i>capitata</i>), but the detailed mechanism underlying leaf color formation remains unclear. In this study, we characterized a <i>Brassica oleracea yellow-green leaf 2</i> (<i>BoYgl-2</i>) mutant 4036Y, which has significantly reduced chlorophyll content and abnormal chloroplasts during early leaf development. Genetic analysis revealed that the yellow-green leaf trait is controlled by a single recessive gene. Map-based cloning revealed that <i>BoYgl-2</i> encodes a novel nuclear-targeted P-type PPR protein, which is absent in the 4036Y mutant. Functional complementation showed that <i>BoYgl-2</i> from the normal-green leaf 4036G can rescue the yellow-green leaf phenotype of 4036Y. The C-to-U editing efficiency and expression levels of <i>atpF</i>, <i>rps14</i>, <i>petL</i> and <i>ndhD</i> were significantly reduced in 4036Y than that in 4036G, and significantly increased in <i>BoYgl-2</i> overexpression lines than that in 4036Y. The expression levels of many plastid- and nuclear-encoded genes associated with chloroplast development in <i>BoYgl-2</i> mutant were also significantly altered. These results suggest that <i>BoYgl-2</i> participates in chloroplast C-to-U editing and development, which provides rare insight into the molecular mechanism underlying leaf color formation in cabbage.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-19T10:14:20.342Z","creation":"2025-04-19T10:14:20.342Z"},"accession":"S-EPMC10980974","cross_references":{"pubmed":["38559470"],"doi":["10.1093/hr/uhae006"]}}