biostudies-literatureUnknown11(3)Zhang BLeaf color is an important agronomic trait in cabbage (<i>Brassica oleracea</i> L. var. <i>capitata</i>), but the detailed mechanism underlying leaf color formation remains unclear. In this study, we characterized a <i>Brassica oleracea yellow-green leaf 2</i> (<i>BoYgl-2</i>) mutant 4036Y, which has significantly reduced chlorophyll content and abnormal chloroplasts during early leaf development. Genetic analysis revealed that the yellow-green leaf trait is controlled by a single recessive gene. Map-based cloning revealed that <i>BoYgl-2</i> encodes a novel nuclear-targeted P-type PPR protein, which is absent in the 4036Y mutant. Functional complementation showed that <i>BoYgl-2</i> from the normal-green leaf 4036G can rescue the yellow-green leaf phenotype of 4036Y. The C-to-U editing efficiency and expression levels of <i>atpF</i>, <i>rps14</i>, <i>petL</i> and <i>ndhD</i> were significantly reduced in 4036Y than that in 4036G, and significantly increased in <i>BoYgl-2</i> overexpression lines than that in 4036Y. The expression levels of many plastid- and nuclear-encoded genes associated with chloroplast development in <i>BoYgl-2</i> mutant were also significantly altered. These results suggest that <i>BoYgl-2</i> participates in chloroplast C-to-U editing and development, which provides rare insight into the molecular mechanism underlying leaf color formation in cabbage.Horticulture researchuhae006https://www.ebi.ac.uk/biostudies/studies/S-EPMC10980974biostudies-literatureChloroplast C-to-U editing, regulated by a PPR protein BoYgl-2, is important for chlorophyll biosynthesis in cabbage.PMC10980974Ren WZhuang MLi SYang LLv HZhang YJi JWang YHou XZhang BWu YfalseChloroplast C-to-U editing, regulated by a PPR protein BoYgl-2, is important for chlorophyll biosynthesis in cabbage.Leaf color is an important agronomic trait in cabbage (<i>Brassica oleracea</i> L. var. <i>capitata</i>), but the detailed mechanism underlying leaf color formation remains unclear. In this study, we characterized a <i>Brassica oleracea yellow-green leaf 2</i> (<i>BoYgl-2</i>) mutant 4036Y, which has significantly reduced chlorophyll content and abnormal chloroplasts during early leaf development. Genetic analysis revealed that the yellow-green leaf trait is controlled by a single recessive gene. Map-based cloning revealed that <i>BoYgl-2</i> encodes a novel nuclear-targeted P-type PPR protein, which is absent in the 4036Y mutant. Functional complementation showed that <i>BoYgl-2</i> from the normal-green leaf 4036G can rescue the yellow-green leaf phenotype of 4036Y. The C-to-U editing efficiency and expression levels of <i>atpF</i>, <i>rps14</i>, <i>petL</i> and <i>ndhD</i> were significantly reduced in 4036Y than that in 4036G, and significantly increased in <i>BoYgl-2</i> overexpression lines than that in 4036Y. The expression levels of many plastid- and nuclear-encoded genes associated with chloroplast development in <i>BoYgl-2</i> mutant were also significantly altered. These results suggest that <i>BoYgl-2</i> participates in chloroplast C-to-U editing and development, which provides rare insight into the molecular mechanism underlying leaf color formation in cabbage.2024-01-01T00:00:00Z2024 Mar2025-04-19T10:14:20.342Z2025-04-19T10:14:20.342ZS-EPMC109809743855947010.1093/hr/uhae006