{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rodriguez-Castro L"],"funding":["ANID Programa de Investigación Asociativa","Núcleo Milenio Bioproductos, Genómica y Microbiología Ambiental","USM","FONDECYT"],"pagination":["12"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10983741"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["57(1)"],"pubmed_abstract":["Background
Bacterial aromatic degradation may cause oxidative stress. The long-chain flavodoxin FldX1 of Paraburkholderia xenovorans LB400 counteracts reactive oxygen species (ROS). The aim of this study was to evaluate the protective role of FldX1 in P. xenovorans LB400 during the degradation of 4-hydroxyphenylacetate (4-HPA) and 3-hydroxyphenylacetate (3-HPA).Methods
The functionality of FldX1 was evaluated in P. xenovorans p2-fldX1 that overexpresses FldX1. The effects of FldX1 on P. xenovorans were studied measuring growth on hydroxyphenylacetates, degradation of 4-HPA and 3-HPA, and ROS formation. The effects of hydroxyphenylacetates (HPAs) on the proteome (LC-MS/MS) and gene expression (qRT-PCR) were quantified. Bioaugmentation with strain p2-fldX1 of 4-HPA-polluted s"],"journal":["Biological research"],"pubmed_title":["The long-chain flavodoxin FldX1 improves the biodegradation of 4-hydroxyphenylacetate and 3-hydroxyphenylacetate and counteracts the oxidative stress associated to aromatic catabolism in Paraburkholderia xenovorans."],"pmcid":["PMC10983741"],"funding_grant_id":["PI_ PI_M_23_02","1200756","Anillo GAMBIO ACT172128","NCN2023_054"],"pubmed_authors":["Rodriguez-Castro L","Riedel K","Zuhlke D","Seeger M","Mendez V","Dorochesi F","Duran RE"],"additional_accession":[]},"is_claimable":false,"name":"The long-chain flavodoxin FldX1 improves the biodegradation of 4-hydroxyphenylacetate and 3-hydroxyphenylacetate and counteracts the oxidative stress associated to aromatic catabolism in Paraburkholderia xenovorans.","description":"Background
Bacterial aromatic degradation may cause oxidative stress. The long-chain flavodoxin FldX1 of Paraburkholderia xenovorans LB400 counteracts reactive oxygen species (ROS). The aim of this study was to evaluate the protective role of FldX1 in P. xenovorans LB400 during the degradation of 4-hydroxyphenylacetate (4-HPA) and 3-hydroxyphenylacetate (3-HPA).Methods
The functionality of FldX1 was evaluated in P. xenovorans p2-fldX1 that overexpresses FldX1. The effects of FldX1 on P. xenovorans were studied measuring growth on hydroxyphenylacetates, degradation of 4-HPA and 3-HPA, and ROS formation. The effects of hydroxyphenylacetates (HPAs) on the proteome (LC-MS/MS) and gene expression (qRT-PCR) were quantified. Bioaugmentation with strain p2-fldX1 of 4-HPA-polluted s","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-06-01T23:45:40.99Z","creation":"2024-10-19T02:15:51.694Z"},"accession":"S-EPMC10983741","cross_references":{"pubmed":["38561836"],"doi":["10.1186/s40659-024-00491-4"]}}