{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pei W"],"funding":["National Key R&amp;D Program of China","National Key R&D Program of China","National Natural Science Foundation of China"],"pagination":["96"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10988907"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["<h4>Background</h4>Ovarian ageing is one of the major issues that impacts female fertility. Mesenchymal stem cell (MSC)-based therapy has made impressive progress in recent years. However, the efficacy and safety of MSCs, as nonautologous components, remain to be further verified.<h4>Methods</h4>Two common sources of MSCs, umbilical cord-derived MSCs (UC-MSCs) and adipose tissue-derived MSCs (AD-MSCs), were orthotopically transplanted into a mouse model of ovarian ageing to evaluate their therapeutic effects. The safety of the treatment was further evaluated, and RNA sequencing was performed to explore the underlying mechanisms involved.<h4>Results</h4>After orthotopic transplantation of MSCs into the ovary, the oestrous cycle, ovarian weight, number and proportion of primary follicles, granulosa cell proliferation, and angiogenesis were improved. The effects of AD-MSCs were superior to those of UC-MSCs in several indices, such as post-transplant granulosa cell proliferation, ovarian weight and angiogenesis. Moreover, the tumorigenesis, acute toxicity, immunogenicity and biodistribution of MSCs were evaluated, and both AD-MSCs and UC-MSCs were found to possess high safety profiles. Through RNA sequencing analysis, enhancement of the MAPK cascade was observed, and long-term effects were mainly linked to the activation of immune function.<h4>Conclusions</h4>Orthotopic transplantation of MSCs displays significant efficacy and high safety for the treatment of ovarian ageing in mice."],"journal":["Stem cell research & therapy"],"pubmed_title":["Efficacy and safety of mesenchymal stem cell therapy for ovarian ageing in a mouse model."],"pmcid":["PMC10988907"],"funding_grant_id":["82288102","82192873","82225019","2021YFC2700303"],"pubmed_authors":["Fan Y","Guo W","Fu L","Yang R","Li R","Wang Y","Pei W","Qiao J","Yu Y"],"additional_accession":[]},"is_claimable":false,"name":"Efficacy and safety of mesenchymal stem cell therapy for ovarian ageing in a mouse model.","description":"<h4>Background</h4>Ovarian ageing is one of the major issues that impacts female fertility. Mesenchymal stem cell (MSC)-based therapy has made impressive progress in recent years. However, the efficacy and safety of MSCs, as nonautologous components, remain to be further verified.<h4>Methods</h4>Two common sources of MSCs, umbilical cord-derived MSCs (UC-MSCs) and adipose tissue-derived MSCs (AD-MSCs), were orthotopically transplanted into a mouse model of ovarian ageing to evaluate their therapeutic effects. The safety of the treatment was further evaluated, and RNA sequencing was performed to explore the underlying mechanisms involved.<h4>Results</h4>After orthotopic transplantation of MSCs into the ovary, the oestrous cycle, ovarian weight, number and proportion of primary follicles, granulosa cell proliferation, and angiogenesis were improved. The effects of AD-MSCs were superior to those of UC-MSCs in several indices, such as post-transplant granulosa cell proliferation, ovarian weight and angiogenesis. Moreover, the tumorigenesis, acute toxicity, immunogenicity and biodistribution of MSCs were evaluated, and both AD-MSCs and UC-MSCs were found to possess high safety profiles. Through RNA sequencing analysis, enhancement of the MAPK cascade was observed, and long-term effects were mainly linked to the activation of immune function.<h4>Conclusions</h4>Orthotopic transplantation of MSCs displays significant efficacy and high safety for the treatment of ovarian ageing in mice.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2025-04-18T20:13:45.391Z","creation":"2025-04-07T08:04:56.801Z"},"accession":"S-EPMC10988907","cross_references":{"pubmed":["38570892"],"doi":["10.1186/s13287-024-03698-0"]}}