biostudies-literatureGong ZNational Natural Science Foundation of China9https://www.ebi.ac.uk/biostudies/studies/S-EPMC10991106biostudies-literatureUnknown8(1)Lignin deposits formed on the surface of pretreated lignocellulosic substrates during acidic pretreatments can non-productively adsorb costly enzymes and thereby influence the enzymatic hydrolysis efficiency of cellulose. In this article, peanut protein (PP), a biocompatible non-catalytic protein, was separated from defatted peanut flour (DPF) as a lignin blocking additive to overcome this adverse effect. With the addition of 2.5 g/L PP in enzymatic hydrolysis medium, the glucose yield of the bamboo substrate pretreated by phenylsulfonic acid (PSA) significantly increased from 38 to 94% at a low cellulase loading of 5 FPU/g glucan while achieving a similar glucose yield required a cellulase loading of 17.5 FPU/g glucan without PP addition. Similar promotion effects were also observed on thBioresources and bioprocessingUnderstanding the promoting effect of non-catalytic protein on enzymatic hydrolysis efficiency of lignocelluloses.PMC10991106320717163187055931901262Yang GLiu JChen LZheng PZhu WHuang LLuo XGong ZSong JShuai LfalseUnderstanding the promoting effect of non-catalytic protein on enzymatic hydrolysis efficiency of lignocelluloses.Lignin deposits formed on the surface of pretreated lignocellulosic substrates during acidic pretreatments can non-productively adsorb costly enzymes and thereby influence the enzymatic hydrolysis efficiency of cellulose. In this article, peanut protein (PP), a biocompatible non-catalytic protein, was separated from defatted peanut flour (DPF) as a lignin blocking additive to overcome this adverse effect. With the addition of 2.5 g/L PP in enzymatic hydrolysis medium, the glucose yield of the bamboo substrate pretreated by phenylsulfonic acid (PSA) significantly increased from 38 to 94% at a low cellulase loading of 5 FPU/g glucan while achieving a similar glucose yield required a cellulase loading of 17.5 FPU/g glucan without PP addition. Similar promotion effects were also observed on th2021-01-01T00:00:00Z2021 Jan2026-07-15T02:51:30.42Z2026-06-28T03:06:46.793ZS-EPMC109911063865018210.1186/s40643-021-00363-9