{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Maier JL"],"funding":["NIAID NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["<i>Salmonella enterica</i> Serovar Typhimurium (<i>Salmonella</i>) and its bacteriophage P22 are a model system for the study of horizontal gene transfer by generalized transduction. Typically, the P22 DNA packaging machinery initiates packaging when a short sequence of DNA, known as the pac site, is recognized on the P22 genome. However, sequences similar to the pac site in the host genome, called pseudo-pac sites, lead to erroneous packaging and subsequent generalized transduction of <i>Salmonella</i> DNA. While the general genomic locations of the <i>Salmonella</i> pseudo-pac sites are known, the sequences themselves have not been determined. We used visualization of P22 sequencing reads mapped to host <i>Salmonella</i> genomes to define regions of generalized transduction initiation an"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.03.25.586692"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10996546"],"repository":["biostudies-literature"],"pubmed_title":["Pseudo-pac site sequences used by phage P22 in generalized transduction of <i>Salmonella</i>."],"pmcid":["PMC10996546"],"funding_grant_id":["R01 AI141479","R35 GM138362"],"pubmed_authors":["Gin C","Maier JL","Callahan B","Kleiner M","Sheriff EK","Duerkop BA"],"additional_accession":[]},"is_claimable":false,"name":"Pseudo-pac site sequences used by phage P22 in generalized transduction of <i>Salmonella</i>.","description":"<i>Salmonella enterica</i> Serovar Typhimurium (<i>Salmonella</i>) and its bacteriophage P22 are a model system for the study of horizontal gene transfer by generalized transduction. Typically, the P22 DNA packaging machinery initiates packaging when a short sequence of DNA, known as the pac site, is recognized on the P22 genome. However, sequences similar to the pac site in the host genome, called pseudo-pac sites, lead to erroneous packaging and subsequent generalized transduction of <i>Salmonella</i> DNA. While the general genomic locations of the <i>Salmonella</i> pseudo-pac sites are known, the sequences themselves have not been determined. We used visualization of P22 sequencing reads mapped to host <i>Salmonella</i> genomes to define regions of generalized transduction initiation an","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-26T20:09:01.881Z","creation":"2025-04-06T16:20:32.101Z"},"accession":"S-EPMC10996546","cross_references":{"pubmed":["38585963"],"doi":["10.1101/2024.03.25.586692"]}}