biostudies-literatureUnknownMaier JLNIAID NIH HHSNIGMS NIH HHS<i>Salmonella enterica</i> Serovar Typhimurium (<i>Salmonella</i>) and its bacteriophage P22 are a model system for the study of horizontal gene transfer by generalized transduction. Typically, the P22 DNA packaging machinery initiates packaging when a short sequence of DNA, known as the pac site, is recognized on the P22 genome. However, sequences similar to the pac site in the host genome, called pseudo-pac sites, lead to erroneous packaging and subsequent generalized transduction of <i>Salmonella</i> DNA. While the general genomic locations of the <i>Salmonella</i> pseudo-pac sites are known, the sequences themselves have not been determined. We used visualization of P22 sequencing reads mapped to host <i>Salmonella</i> genomes to define regions of generalized transduction initiation anbioRxiv : the preprint server for biology2024.03.25.586692https://www.ebi.ac.uk/biostudies/studies/S-EPMC10996546biostudies-literaturePseudo-pac site sequences used by phage P22 in generalized transduction of <i>Salmonella</i>.PMC10996546R01 AI141479R35 GM138362Gin CMaier JLCallahan BKleiner MSheriff EKDuerkop BAfalsePseudo-pac site sequences used by phage P22 in generalized transduction of <i>Salmonella</i>.<i>Salmonella enterica</i> Serovar Typhimurium (<i>Salmonella</i>) and its bacteriophage P22 are a model system for the study of horizontal gene transfer by generalized transduction. Typically, the P22 DNA packaging machinery initiates packaging when a short sequence of DNA, known as the pac site, is recognized on the P22 genome. However, sequences similar to the pac site in the host genome, called pseudo-pac sites, lead to erroneous packaging and subsequent generalized transduction of <i>Salmonella</i> DNA. While the general genomic locations of the <i>Salmonella</i> pseudo-pac sites are known, the sequences themselves have not been determined. We used visualization of P22 sequencing reads mapped to host <i>Salmonella</i> genomes to define regions of generalized transduction initiation an2024-01-01T00:00:00Z2024 Mar2025-04-26T20:09:01.881Z2025-04-06T16:20:32.101ZS-EPMC109965463858596310.1101/2024.03.25.586692