{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zou J"],"funding":["Human Frontier Science Program","U.S. Department of Health &amp; Human Services | NIH | National Institute of General Medical Sciences","U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS)","United States Department of Defense | United States Air Force | AFMC | Air Force Office of Scientific Research","NHLBI NIH HHS","U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS)","U.S. Department of Health &amp; Human Services | NIH | National Heart, Lung, and Blood Institute","United States Department of Defense | United States Air Force | AFMC | Air Force Office of Scientific Research (AF Office of Scientific Research)","U.S. Department of Health &amp; Human Services | NIH | National Institute of Neurological Disorders and Stroke","NINDS NIH HHS","Human Frontier Science Program (HFSP)","U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI)","NIGMS NIH HHS"],"pagination":["1075-1083"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11004682"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["42(7)"],"pubmed_abstract":["Cellular sodium ion (Na<sup>+</sup>) homeostasis is integral to organism physiology. Our current understanding of Na<sup>+</sup> homeostasis is largely limited to Na<sup>+</sup> transport at the plasma membrane. Organelles may also contribute to Na<sup>+</sup> homeostasis; however, the direction of Na<sup>+</sup> flow across organelle membranes is unknown because organellar Na<sup>+</sup> cannot be imaged. Here we report a pH-independent, organelle-targetable, ratiometric probe that reports lumenal Na<sup>+</sup>. It is a DNA nanodevice containing a Na<sup>+</sup>-sensitive fluorophore, a reference dye and an organelle-targeting domain. By measuring Na<sup>+</sup> at single endosome resolution in mammalian cells and Caenorhabditis elegans, we discovered that lumenal Na<sup>+</sup> levels i"],"journal":["Nature biotechnology"],"pubmed_title":["A DNA nanodevice for mapping sodium at single-organelle resolution."],"pmcid":["PMC11004682"],"funding_grant_id":["RGP0032/2022","DP1 GM149751","R01 GM147197","1R01NS112139-01A1","R21 HL161825","FA9550-19-0003","R01 NS112139","1R01GM147197-01","R21HL161825-01A1","DP1GM149751","R21 NS114428","1R21NS114428-01"],"pubmed_authors":["Zou J","Rao R","Krishnan Y","Smith JJ","Mitra K","Veetil AT","Oettinger D","Ramirez JR","Anees P","Gupta PD","Kratsios P"],"additional_accession":[]},"is_claimable":false,"name":"A DNA nanodevice for mapping sodium at single-organelle resolution.","description":"Cellular sodium ion (Na<sup>+</sup>) homeostasis is integral to organism physiology. Our current understanding of Na<sup>+</sup> homeostasis is largely limited to Na<sup>+</sup> transport at the plasma membrane. Organelles may also contribute to Na<sup>+</sup> homeostasis; however, the direction of Na<sup>+</sup> flow across organelle membranes is unknown because organellar Na<sup>+</sup> cannot be imaged. Here we report a pH-independent, organelle-targetable, ratiometric probe that reports lumenal Na<sup>+</sup>. It is a DNA nanodevice containing a Na<sup>+</sup>-sensitive fluorophore, a reference dye and an organelle-targeting domain. By measuring Na<sup>+</sup> at single endosome resolution in mammalian cells and Caenorhabditis elegans, we discovered that lumenal Na<sup>+</sup> levels i","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2025-04-04T15:02:11.93Z","creation":"2025-04-04T15:02:11.93Z"},"accession":"S-EPMC11004682","cross_references":{"pubmed":["37735265"],"doi":["10.1038/s41587-023-01950-1"]}}