biostudies-literaturePees BDeutsche ForschungsgemeinschaftNIH HHSe0346323https://www.ebi.ac.uk/biostudies/studies/S-EPMC11005407biostudies-literatureUnknown15(4)The <i>Caenorhabditis elegans</i> natural microbiota isolates <i>Pseudomonas lurida</i> MYb11 and <i>Pseudomonas fluorescens</i> MYb115 protect the host against pathogens through distinct mechanisms. While <i>P. lurida</i> produces an antimicrobial compound and directly inhibits pathogen growth, <i>P. fluorescens</i> MYb115 protects the host without affecting pathogen growth. It is unknown how these two protective microbes affect host biological processes. We used a proteomics approach to elucidate the <i>C. elegans</i> response to MYb11 and MYb115. We found that both <i>Pseudomonas</i> isolates increase vitellogenin protein production in young adults, which confirms previous findings on the effect of microbiota on <i>C. elegans</i> reproductive timing. Moreover, the <i>C. elegans</i> respmBioThe <i>Caenorhabditis elegans</i> proteome response to two protective <i>Pseudomonas</i> symbionts.PMC11005407P40 OD010440CRC1182 Project A1.2, CRC1182 Project Z3Tholey AHamerich IKDierking KPees BPeters LTreitz CKissoyan KABfalseThe <i>Caenorhabditis elegans</i> proteome response to two protective <i>Pseudomonas</i> symbionts.The <i>Caenorhabditis elegans</i> natural microbiota isolates <i>Pseudomonas lurida</i> MYb11 and <i>Pseudomonas fluorescens</i> MYb115 protect the host against pathogens through distinct mechanisms. While <i>P. lurida</i> produces an antimicrobial compound and directly inhibits pathogen growth, <i>P. fluorescens</i> MYb115 protects the host without affecting pathogen growth. It is unknown how these two protective microbes affect host biological processes. We used a proteomics approach to elucidate the <i>C. elegans</i> response to MYb11 and MYb115. We found that both <i>Pseudomonas</i> isolates increase vitellogenin protein production in young adults, which confirms previous findings on the effect of microbiota on <i>C. elegans</i> reproductive timing. Moreover, the <i>C. elegans</i> resp2024-01-01T00:00:00Z2024 Apr2026-05-29T12:39:48.196Z2024-11-19T19:41:43.747ZS-EPMC110054073841107810.1128/mbio.03463-23