biostudies-literatureStrauss MSecretaría de Ciencia y Tecnología, Universidad Nacional de Córdoba, ArgentinaFONCyT, Argentina1595-1601https://www.ebi.ac.uk/biostudies/studies/S-EPMC11010057biostudies-literatureUnknown148(13)The aim of the present work was to evaluate the distribution of the different clones of the parasite prevailing after treatment with benznidazole (BZ) and clomipramine (CLO), in mice infected with Trypanosoma cruzi, Casibla isolate which consists of a mixture of two discrete typing units (DTUs). Albino Swiss mice were infected and treated with high and low concentrations of BZ (100 or 6.25 mg/kg), CLO (5 or 1.25 mg/kg), or the combination of both low doses (BZ6.25 + CLO1.25), during the acute phase of experimental infection. Treatment efficacy was evaluated by comparing parasitaemia, survival and tissular parasite presence. For DTUs genotyping, blood, skeletal and cardiac muscle samples were analysed by multiplex quantitative polymerase chain reaction. The combined treatment had similar ouParasitologyDifferential tissue distribution of discrete typing units after drug combination therapy in experimental <i>Trypanosoma cruzi</i> mixed infection.PMC11010057PICT 2014-11882012-2013 and 2014-2015Paglini PALo Presti MSSchijman AGRivarola HWStrauss MBazan PCVelazquez Lopez DARamirez JCBaez ALfalseDifferential tissue distribution of discrete typing units after drug combination therapy in experimental <i>Trypanosoma cruzi</i> mixed infection.The aim of the present work was to evaluate the distribution of the different clones of the parasite prevailing after treatment with benznidazole (BZ) and clomipramine (CLO), in mice infected with Trypanosoma cruzi, Casibla isolate which consists of a mixture of two discrete typing units (DTUs). Albino Swiss mice were infected and treated with high and low concentrations of BZ (100 or 6.25 mg/kg), CLO (5 or 1.25 mg/kg), or the combination of both low doses (BZ6.25 + CLO1.25), during the acute phase of experimental infection. Treatment efficacy was evaluated by comparing parasitaemia, survival and tissular parasite presence. For DTUs genotyping, blood, skeletal and cardiac muscle samples were analysed by multiplex quantitative polymerase chain reaction. The combined treatment had similar ou2021-01-01T00:00:00Z2021 Nov2025-04-04T09:34:07.071Z2025-04-04T09:34:07.071ZS-EPMC110100573506046810.1017/S003118202100128110.1017/s0031182021001281