biostudies-literatureCreighton RLNSFEunice Kennedy Shriver National Institute of Child Health and Human DevelopmentNICHD NIH HHSNIDCR NIH HHSNIAID NIH HHSNational Institutes of HealthNIHMolecular Engineering and Sciences Institute, University of WashingtonUniversity of WashingtonNational Institute of Dental and Craniofacial ResearchWashington Research FoundationClean Energy InstituteNational Science Foundation649-660https://www.ebi.ac.uk/biostudies/studies/S-EPMC11010722biostudies-literatureUnknown367The oral mucosa is an attractive site for immunization due to its accessibility and ability to elicit local and systemic immune responses. However, evaluating oral mucosal immunogenicity has proven challenging due to the physical barriers and immunological complexity of the oral mucosa. Microneedles can overcome these physical barriers, but previous work has been limited in the scope of microneedle delivery site, geometry, and release kinetics, all of which are expected to affect physiological responses. Here, we develop integrated fiber microneedle devices, an oral dosage form with tunable geometries and material configurations capable of both burst and sustained release to controlled depths in the oral mucosa. Integrated fiber microneedles administered to either the buccal or sublingual Journal of controlled release : official journal of the Controlled Release SocietyOral mucosal vaccination using integrated fiber microneedles.PMC11010722DP2 HD075703DGE-1256082T32 AI007140DP2HD075703R56 DE0285391R56DE0285392T32AI007140-41Doan MAWoodrow KACreighton RLTobos CIGuo TFaber KAfalseOral mucosal vaccination using integrated fiber microneedles.The oral mucosa is an attractive site for immunization due to its accessibility and ability to elicit local and systemic immune responses. However, evaluating oral mucosal immunogenicity has proven challenging due to the physical barriers and immunological complexity of the oral mucosa. Microneedles can overcome these physical barriers, but previous work has been limited in the scope of microneedle delivery site, geometry, and release kinetics, all of which are expected to affect physiological responses. Here, we develop integrated fiber microneedle devices, an oral dosage form with tunable geometries and material configurations capable of both burst and sustained release to controlled depths in the oral mucosa. Integrated fiber microneedles administered to either the buccal or sublingual 2024-01-01T00:00:00Z2024 Mar2025-04-04T10:42:12.404Z2025-04-04T10:42:12.404ZS-EPMC110107223829599310.1016/j.jconrel.2024.01.062