biostudies-literatureImomnazarov KAlzheimer’s AssociationCenter for Translational Research in Neurodegenerative DiseaseNIH NINDSAligning Science Across Parkinson’sParkinson’s FoundationAlzheimer's AssociationNIH HHS3643https://www.ebi.ac.uk/biostudies/studies/S-EPMC11011978biostudies-literatureUnknown25(7)Synucleinopathies are a group of central nervous system pathologies that are characterized by the intracellular accumulation of misfolded and aggregated α-synuclein in proteinaceous depositions known as Lewy Bodies (LBs). The transition of α-synuclein from its physiological to pathological form has been associated with several post-translational modifications such as phosphorylation and an increasing degree of insolubility, which also correlate with disease progression in post-mortem specimens from human patients. Neuronal expression of α-synuclein in model organisms, including <i>Drosophila melanogaster</i>, has been a typical approach employed to study its physiological effects. Biochemical analysis of α-synuclein solubility via high-speed ultracentrifugation with buffers of increasing dInternational journal of molecular sciencesBiochemical Fractionation of Human α-Synuclein in a <i>Drosophila</i> Model of Synucleinopathies.PMC11011978AARG-D-22-972117AARG-D-22–972117P40 OD0185371RF1NS28800ASAP-020527Lopez-Scarim JBagheri IMartin-Pena ATansey MGImomnazarov KJoers VfalseBiochemical Fractionation of Human α-Synuclein in a <i>Drosophila</i> Model of Synucleinopathies.Synucleinopathies are a group of central nervous system pathologies that are characterized by the intracellular accumulation of misfolded and aggregated α-synuclein in proteinaceous depositions known as Lewy Bodies (LBs). The transition of α-synuclein from its physiological to pathological form has been associated with several post-translational modifications such as phosphorylation and an increasing degree of insolubility, which also correlate with disease progression in post-mortem specimens from human patients. Neuronal expression of α-synuclein in model organisms, including <i>Drosophila melanogaster</i>, has been a typical approach employed to study its physiological effects. Biochemical analysis of α-synuclein solubility via high-speed ultracentrifugation with buffers of increasing d2024-01-01T00:00:00Z2024 Mar2026-04-21T03:29:07.096Z2025-04-06T16:20:54.563ZS-EPMC110119783861245410.3390/ijms25073643