{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Abebe JS"],"funding":["NIAID NIH HHS"],"pubmed_abstract":["High-resolution annotations of transcriptomes from all domains of life are essential for many sequencing-based RNA analyses, including Nanopore direct RNA sequencing (DRS), which would otherwise be hindered by misalignments and other analysis artefacts. DRS allows the capture and full-length sequencing of native RNAs, without recoding or amplification bias, and resulting data may be interrogated to define the identity and location of chemically modified ribonucleotides, as well as the length of poly(A) tails on individual RNA molecules. Existing software solutions for generating high-resolution transcriptome annotations are poorly suited to small gene dense organisms such as viruses due to the challenge of identifying distinct transcript isoforms where alternative splicing and overlapping "],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.04.02.587744"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11014626"],"repository":["biostudies-literature"],"pubmed_title":["Nanopore Guided Annotation of Transcriptome Architectures."],"pmcid":["PMC11014626"],"funding_grant_id":["R01 AI152543","R01 AI170583","R01 AI176335"],"pubmed_authors":["Wilson AC","Leins J","Fuhrmann E","Mai J","Abebe JS","Verstraten R","Depledge DP","Alwie Y","Schreiner S"],"additional_accession":[]},"is_claimable":false,"name":"Nanopore Guided Annotation of Transcriptome Architectures.","description":"High-resolution annotations of transcriptomes from all domains of life are essential for many sequencing-based RNA analyses, including Nanopore direct RNA sequencing (DRS), which would otherwise be hindered by misalignments and other analysis artefacts. DRS allows the capture and full-length sequencing of native RNAs, without recoding or amplification bias, and resulting data may be interrogated to define the identity and location of chemically modified ribonucleotides, as well as the length of poly(A) tails on individual RNA molecules. Existing software solutions for generating high-resolution transcriptome annotations are poorly suited to small gene dense organisms such as viruses due to the challenge of identifying distinct transcript isoforms where alternative splicing and overlapping ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-05-21T03:09:02.608Z","creation":"2026-05-21T03:08:09.018Z"},"accession":"S-EPMC11014626","cross_references":{"pubmed":["38617228"],"doi":["10.1101/2024.04.02.587744"]}}