biostudies-literatureGasca-Capote CConsejería de Economía, Conocimiento, Empresas y Universidad, Junta de AndalucíaInstituto de Salud Carlos IIINIDDK NIH HHSNIDA NIH HHSNIAID NIH HHSNIMH NIH HHSNHLBI NIH HHSJunta de AndalucíaGilead Fellowshipse174215https://www.ebi.ac.uk/biostudies/studies/S-EPMC11014653biostudies-literatureUnknown134(8)BACKGROUNDPersistent controllers (PCs) maintain antiretroviral-free HIV-1 control indefinitely over time, while transient controllers (TCs) eventually lose virological control. It is essential to characterize the quality of the HIV reservoir in terms of these phenotypes in order to identify the factors that lead to HIV progression and to open new avenues toward an HIV cure.METHODSThe characterization of HIV-1 reservoir from peripheral blood mononuclear cells was performed using next-generation sequencing techniques, such as full-length individual and matched integration site proviral sequencing (FLIP-Seq; MIP-Seq).RESULTSPCs and TCs, before losing virological control, presented significantly lower total, intact, and defective proviruses compared with those of participants on antiretroviralThe Journal of clinical investigationThe HIV-1 reservoir landscape in persistent elite controllers and transient elite controllers.PMC11014653R33 DA047034R01 AI130005U01 AI135940U01 AI117841R01 AI176579UM1 AI164570R01 DK120387R01 MH134823UM1 AI164560R61 DA047034R37 AI155171GLD22/00147R01 HL134539UM1 AI164566R21 AI116228UM1 AI164562US-75 1380938R01 AI078799R33 AI116228R01 AI152979FI17/00186,FI19/00083,MV20/00057,PI18/01532,PI19/01127,PI22/01796PI20/1276K24 AI155233Peraire JRivero-Juarez ARafii-El-Idrissi Benhnia MGallego IEspinosa NYu XGCamacho-Sojo MIPalacios RSantos JRivero ALopez-Ruz MAGao COlalla JBachiller SHidalgo-Tenorio CRull APerreau MMunoz-Medina LMastrangelo ALopez-Cortes LFLopez-Cortes LECavassini MGasca-Capote CVitalle JVilades CPerez-Gomez AGladkov GLian XCollado-Romacho ARFrias MRuiz-Mateos ERuiz-Sancho AVidal FRoseto ICJimenez-Leon MRGomez-Ayerbe CLichterfeld MOstos FJRoca-Oporto CfalseThe HIV-1 reservoir landscape in persistent elite controllers and transient elite controllers.BACKGROUNDPersistent controllers (PCs) maintain antiretroviral-free HIV-1 control indefinitely over time, while transient controllers (TCs) eventually lose virological control. It is essential to characterize the quality of the HIV reservoir in terms of these phenotypes in order to identify the factors that lead to HIV progression and to open new avenues toward an HIV cure.METHODSThe characterization of HIV-1 reservoir from peripheral blood mononuclear cells was performed using next-generation sequencing techniques, such as full-length individual and matched integration site proviral sequencing (FLIP-Seq; MIP-Seq).RESULTSPCs and TCs, before losing virological control, presented significantly lower total, intact, and defective proviruses compared with those of participants on antiretroviral2024-01-01T00:00:00Z2024 Feb2026-06-04T05:30:08.934Z2025-04-19T20:29:30.216ZS-EPMC110146533837691810.1172/JCI174215