biostudies-literatureTurkowski KDeutsche Forschungsgemeinschaft (German Research Foundation)1178-1189https://www.ebi.ac.uk/biostudies/studies/S-EPMC11014796biostudies-literatureUnknown43(16)Dual-specificity phosphatase 8 (DUSP8) plays an important role as a selective c-Jun N-terminal kinase (JNK) phosphatase in mitogen-activated protein kinase (MAPK) signaling. In this study, we found that DUSP8 is silenced by miR-147b in patients with lung adenocarcinoma (LUAD), which correlates with poor overall survival. Overexpression of DUSP8 resulted in a tumor-suppressive phenotype in vitro and in vivo experimental models, whereas silencing DUSP8 with a siRNA approach abrogated the tumor-suppressive properties. We found that miR-147b is a posttranscriptional regulator of DUSP8 that is highly expressed in patients with LUAD and is associated with lower survival. NanoString analysis revealed that the MAPK signaling pathway is mainly affected by overexpression of miR-147b, leading to incrOncogenemiR-147b mediated suppression of DUSP8 promotes lung cancer progression.PMC11014796SA 1923/7-1, SFB1213 (Project A01, A05, A10N)Brunn DFink LHerzberg FTurkowski KGrimminger FPullamsetti SSWeigert ASeeger WSultmann HHaselbauer TSavai RStiewe TGunther SfalsemiR-147b mediated suppression of DUSP8 promotes lung cancer progression.Dual-specificity phosphatase 8 (DUSP8) plays an important role as a selective c-Jun N-terminal kinase (JNK) phosphatase in mitogen-activated protein kinase (MAPK) signaling. In this study, we found that DUSP8 is silenced by miR-147b in patients with lung adenocarcinoma (LUAD), which correlates with poor overall survival. Overexpression of DUSP8 resulted in a tumor-suppressive phenotype in vitro and in vivo experimental models, whereas silencing DUSP8 with a siRNA approach abrogated the tumor-suppressive properties. We found that miR-147b is a posttranscriptional regulator of DUSP8 that is highly expressed in patients with LUAD and is associated with lower survival. NanoString analysis revealed that the MAPK signaling pathway is mainly affected by overexpression of miR-147b, leading to incr2024-01-01T00:00:00Z2024 Apr2025-04-19T20:26:32.803Z2025-04-19T20:26:32.803ZS-EPMC110147963839629310.1038/s41388-024-02969-7