biostudies-literatureXu MMinistry of Science and Technology of the People's Republic of ChinaNational Natural Science Foundation of ChinaBiotechnology and Biological Sciences Research Council103693https://www.ebi.ac.uk/biostudies/studies/S-EPMC11017069biostudies-literatureUnknown103(6)Avian leukosis virus subgroup J (ALV-J) is a retrovirus that can cause immunosuppression and tumors in chicken. However, relative pathogenesis is still not clear. At present, metabolomics has shown great potential in the screening of tumor metabolic markers, prognostic evaluation, and drug target design. In this study, we utilize an untargeted metabolomics approach based on ultrahigh-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS) to analyze the metabolic changes in chicken embryo fibroblast (CEF) cells infected by ALV-J. We found that ALV-J infection significantly altered a wealth of metabolites compared with control group. Additionally, most of the differentially expressed metabolites belonged to lipid metabolism, purine nucleotide metPoultry scienceMetabolomics analysis of CEF cells infected with avian leukosis virus subgroup J based on UHPLC-QE-MS.PMC11017069BB/R012865/1Nair VQin AShao HXu MYao YQian KYe JfalseMetabolomics analysis of CEF cells infected with avian leukosis virus subgroup J based on UHPLC-QE-MS.Avian leukosis virus subgroup J (ALV-J) is a retrovirus that can cause immunosuppression and tumors in chicken. However, relative pathogenesis is still not clear. At present, metabolomics has shown great potential in the screening of tumor metabolic markers, prognostic evaluation, and drug target design. In this study, we utilize an untargeted metabolomics approach based on ultrahigh-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS) to analyze the metabolic changes in chicken embryo fibroblast (CEF) cells infected by ALV-J. We found that ALV-J infection significantly altered a wealth of metabolites compared with control group. Additionally, most of the differentially expressed metabolites belonged to lipid metabolism, purine nucleotide met2024-01-01T00:00:00Z2024 Jun2026-04-08T19:41:56.06Z2025-07-25T03:08:00.219ZS-EPMC110170693859891210.1016/j.psj.2024.103693