{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hamel LP"],"funding":["Natural Sciences and Engineering Research Council of Canada","Medicago Inc."],"pagination":["1078-1100"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11022802"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(5)"],"pubmed_abstract":["The production of influenza vaccines in plants is achieved through transient expression of viral hemagglutinins (HAs), a process mediated by the bacterial vector Agrobacterium tumefaciens. HA proteins are then produced and matured through the secretory pathway of plant cells, before being trafficked to the plasma membrane where they induce formation of virus-like particles (VLPs). Production of VLPs unavoidably impacts plant cells, as do viral suppressors of RNA silencing (VSRs) that are co-expressed to increase recombinant protein yields. However, little information is available on host molecular responses to foreign protein expression. This work provides a comprehensive overview of molecular changes occurring in Nicotiana benthamiana leaf cells transiently expressing the VSR P19, or co-e"],"journal":["Plant biotechnology journal"],"pubmed_title":["Molecular responses of agroinfiltrated Nicotiana benthamiana leaves expressing suppressor of silencing P19 and influenza virus-like particles."],"pmcid":["PMC11022802"],"funding_grant_id":["CRDPJ 477619","CRDPJ 495852"],"pubmed_authors":["Comeau MA","Poirier-Gravel F","Rasoolizadeh A","Barrada A","Roussel E","Michaud D","Goulet MC","Brosseau C","D'Aoust MA","Lucier JF","Tardif R","Giroux G","Moffett P","Lavoie PO","Pare ME","Hamel LP"],"additional_accession":[]},"is_claimable":false,"name":"Molecular responses of agroinfiltrated Nicotiana benthamiana leaves expressing suppressor of silencing P19 and influenza virus-like particles.","description":"The production of influenza vaccines in plants is achieved through transient expression of viral hemagglutinins (HAs), a process mediated by the bacterial vector Agrobacterium tumefaciens. HA proteins are then produced and matured through the secretory pathway of plant cells, before being trafficked to the plasma membrane where they induce formation of virus-like particles (VLPs). Production of VLPs unavoidably impacts plant cells, as do viral suppressors of RNA silencing (VSRs) that are co-expressed to increase recombinant protein yields. However, little information is available on host molecular responses to foreign protein expression. This work provides a comprehensive overview of molecular changes occurring in Nicotiana benthamiana leaf cells transiently expressing the VSR P19, or co-e","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-06-01T11:53:35.018Z","creation":"2025-04-22T13:48:54.365Z"},"accession":"S-EPMC11022802","cross_references":{"pubmed":["38041470"],"doi":["10.1111/pbi.14247"]}}