{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sedohara A"],"funding":["The University of Tokyo","the Ministry of Education, Culture, Sports, Science, and Technology of Japan."],"pagination":["103"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11023964"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["169(5)"],"pubmed_abstract":["Missense mutations in certain small envelope proteins diminish the efficacy of antibodies. Consequently, tracking the incidence and types of vaccine-escape mutations (VEMs) was crucial both before and after the introduction of universal hepatitis B vaccination in Japan in 2016. In this study, we isolated hepatitis B virus (HBV) DNA from 58 of 169 hepatitis B surface antigen (HBsAg)-positive blood samples from Japanese blood donors and determined the nucleotide sequence encoding the small envelope protein. DNA from six (10%) of the samples had VEMs, but no missense mutations, such as G145R, were detected. Complete HBV genome sequences were obtained from 29 of the 58 samples; the viral genotype was A1 in one (3%), A2 in three (10%), B1 in nine (31%), B2 in five (17%), B4 in one (3%), and C2 in 10 (34%) samples. Tenofovir-resistance mutations were detected in two (7%) samples. In addition, several core promoter mutations, such as 1762A>T and 1764G>A, and a precore nonsense mutation, 1986G>A, which are risk factors for HBV-related chronic liver disease, were detected. These findings provide a baseline for future research and highlight the importance of ongoing monitoring of VEMs and drug resistance mutations in HBV DNA from HBsAg-positive blood donors without HBV antibodies."],"journal":["Archives of virology"],"pubmed_title":["Characterization of mutations in hepatitis B virus DNA isolated from Japanese HBsAg-positive blood donors in 2021 and 2022."],"pmcid":["PMC11023964"],"funding_grant_id":["000000"],"pubmed_authors":["Tsutsumi T","Yotsuyanagi H","Tuvshinjargal K","Sedohara A","Arizono K","Saito M","Arai K","Nakahara F","Ikeuchi K","Takahashi K","Adachi E"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of mutations in hepatitis B virus DNA isolated from Japanese HBsAg-positive blood donors in 2021 and 2022.","description":"Missense mutations in certain small envelope proteins diminish the efficacy of antibodies. Consequently, tracking the incidence and types of vaccine-escape mutations (VEMs) was crucial both before and after the introduction of universal hepatitis B vaccination in Japan in 2016. In this study, we isolated hepatitis B virus (HBV) DNA from 58 of 169 hepatitis B surface antigen (HBsAg)-positive blood samples from Japanese blood donors and determined the nucleotide sequence encoding the small envelope protein. DNA from six (10%) of the samples had VEMs, but no missense mutations, such as G145R, were detected. Complete HBV genome sequences were obtained from 29 of the 58 samples; the viral genotype was A1 in one (3%), A2 in three (10%), B1 in nine (31%), B2 in five (17%), B4 in one (3%), and C2 in 10 (34%) samples. Tenofovir-resistance mutations were detected in two (7%) samples. In addition, several core promoter mutations, such as 1762A>T and 1764G>A, and a precore nonsense mutation, 1986G>A, which are risk factors for HBV-related chronic liver disease, were detected. These findings provide a baseline for future research and highlight the importance of ongoing monitoring of VEMs and drug resistance mutations in HBV DNA from HBsAg-positive blood donors without HBV antibodies.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-05-19T03:18:59.574Z","creation":"2026-05-19T03:07:27.95Z"},"accession":"S-EPMC11023964","cross_references":{"pubmed":["38632180"],"doi":["10.1007/s00705-024-06016-4"]}}