{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Durinova A"],"funding":["Grantová Agentura, Univerzita Karlova","NETPHARM"],"pagination":["32"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11026318"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(1)"],"pubmed_abstract":["<h4>Background</h4>Megalin (LRP2 receptor) mediates the endocytosis of radiolabeled peptides into proximal tubular kidney cells, which may cause nephrotoxicity due to the accumulation of a radioactive tracer. The study aimed to develop a cellular model of human kidney HK2 cells with LRP2 knockout (KO) using CRISPR/Cas9 technique. This model was employed for the determination of the megalin-mediated accumulation of <sup>68</sup>Ga- and <sup>99m</sup>Tc-labeled 15-mer peptide developed to target the vascular endothelial growth factor (VEGF) receptor in oncology radiodiagnostics.<h4>Results</h4>The gene editing in the LRP2 KO model was verified by testing two well-known megalin ligands when higher viability of KO cells was observed after gentamicin treatment at cytotoxic concentrations and lo"],"journal":["EJNMMI radiopharmacy and chemistry"],"pubmed_title":["Radiolabeled 15-mer peptide internalization is mediated by megalin (LRP2 receptor) in a CRISPR/Cas9-based LRP2 knockout human kidney cell model."],"pmcid":["PMC11026318"],"funding_grant_id":["150120","CZ.02.01.01/00/22_008/0004607"],"pubmed_authors":["Durinova A","Schmierer B","Kamaraj R","Smutna L","Barta P","Pavek P","Smutny T","Trejtnar F"],"additional_accession":[]},"is_claimable":false,"name":"Radiolabeled 15-mer peptide internalization is mediated by megalin (LRP2 receptor) in a CRISPR/Cas9-based LRP2 knockout human kidney cell model.","description":"<h4>Background</h4>Megalin (LRP2 receptor) mediates the endocytosis of radiolabeled peptides into proximal tubular kidney cells, which may cause nephrotoxicity due to the accumulation of a radioactive tracer. The study aimed to develop a cellular model of human kidney HK2 cells with LRP2 knockout (KO) using CRISPR/Cas9 technique. This model was employed for the determination of the megalin-mediated accumulation of <sup>68</sup>Ga- and <sup>99m</sup>Tc-labeled 15-mer peptide developed to target the vascular endothelial growth factor (VEGF) receptor in oncology radiodiagnostics.<h4>Results</h4>The gene editing in the LRP2 KO model was verified by testing two well-known megalin ligands when higher viability of KO cells was observed after gentamicin treatment at cytotoxic concentrations and lo","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-05-29T12:23:35.453Z","creation":"2026-05-19T03:07:31.87Z"},"accession":"S-EPMC11026318","cross_references":{"pubmed":["38637347"],"doi":["10.1186/s41181-024-00262-2"]}}