{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Prytkova I"],"funding":["National Institute of Alcohol Abuse and Alcoholism","NIAAA NIH HHS","NIH HHS"],"pagination":["e0918232024"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11026340"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["44(16)"],"pubmed_abstract":["Genome-wide association studies (GWAS) of electroencephalographic endophenotypes for alcohol use disorder (AUD) has identified noncoding polymorphisms within the <i>KCNJ6</i> gene. <i>KCNJ6</i> encodes GIRK2, a subunit of a G-protein-coupled inwardly rectifying potassium channel that regulates neuronal excitability. We studied the effect of upregulating <i>KCNJ6</i> using an isogenic approach with human glutamatergic neurons derived from induced pluripotent stem cells (male and female donors). Using multielectrode arrays, population calcium imaging, single-cell patch-clamp electrophysiology, and mitochondrial stress tests, we find that elevated GIRK2 acts in concert with 7-21 d of ethanol exposure to inhibit neuronal activity, to counteract ethanol-induced increases in glutamate response, "],"journal":["The Journal of neuroscience : the official journal of the Society for Neuroscience"],"pubmed_title":["Upregulated GIRK2 Counteracts Ethanol-Induced Changes in Excitability and Respiration in Human Neurons."],"pmcid":["PMC11026340"],"funding_grant_id":["S10 OD030463","U10 AA008401","S10 OD026880","F31 AA027949","F31AA027949-01","U10AA008401"],"pubmed_authors":["Hart RP","Prytkova I","Edenberg HJ","Porjesz B","Tischfield JA","Fernando M","Gameiro-Ros I","Popova D","Liu Y","Slesinger PA","Xuei X","Chorlian DB","Kamarajan C","Pang ZP","Goate A"],"additional_accession":[]},"is_claimable":false,"name":"Upregulated GIRK2 Counteracts Ethanol-Induced Changes in Excitability and Respiration in Human Neurons.","description":"Genome-wide association studies (GWAS) of electroencephalographic endophenotypes for alcohol use disorder (AUD) has identified noncoding polymorphisms within the <i>KCNJ6</i> gene. <i>KCNJ6</i> encodes GIRK2, a subunit of a G-protein-coupled inwardly rectifying potassium channel that regulates neuronal excitability. We studied the effect of upregulating <i>KCNJ6</i> using an isogenic approach with human glutamatergic neurons derived from induced pluripotent stem cells (male and female donors). Using multielectrode arrays, population calcium imaging, single-cell patch-clamp electrophysiology, and mitochondrial stress tests, we find that elevated GIRK2 acts in concert with 7-21 d of ethanol exposure to inhibit neuronal activity, to counteract ethanol-induced increases in glutamate response, ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-07-15T05:10:20.628Z","creation":"2026-06-30T03:08:51.932Z"},"accession":"S-EPMC11026340","cross_references":{"pubmed":["38350999"],"doi":["10.1523/JNEUROSCI.0918-23.2024","10.1523/jneurosci.0918-23.2024"]}}