{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15"],"submitter":["Li Q"],"pubmed_abstract":["As an efficient degradation strain, <i>Sphingobium baderi</i> SC-1 can breakdown 3-phenoxybenzoic acid (3-PBA) with high proficiency. To investigate the internal factors that regulate this process, we conducted whole-genome sequencing and successfully identified the pivotal 3-PBA-degrading gene <i>sca</i> (1,230 bp). After <i>sca</i> was expressed in engineered bacteria, a remarkable degradation efficiency was observed, as 20 mg/L 3-PBA was almost completely decomposed within 24 h. The phenol was formed as one of the degradation products. Notably, in addition to their ability to degrade 3-PBA, the resting cells proficiently degraded 4'-HO-3-PBA and 3'-HO-4-PBA. In conclusion, we successfully identified and validated <i>sca</i> as the pivotal enzyme responsible for the efficient degradation"],"journal":["Frontiers in microbiology"],"pagination":["1361335"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11026547"],"repository":["biostudies-literature"],"pubmed_title":["Whole-genome sequencing of <i>Sphingobium baderi</i> SC-1 and identification of a crucial 3-phenoxybenzoic acid-degrading gene."],"pmcid":["PMC11026547"],"pubmed_authors":["Li J","Zhou Q","Zou L","Sarra M","Liu A","Li Q","Chen Y","Hu K","Liu S"],"additional_accession":[]},"is_claimable":false,"name":"Whole-genome sequencing of <i>Sphingobium baderi</i> SC-1 and identification of a crucial 3-phenoxybenzoic acid-degrading gene.","description":"As an efficient degradation strain, <i>Sphingobium baderi</i> SC-1 can breakdown 3-phenoxybenzoic acid (3-PBA) with high proficiency. To investigate the internal factors that regulate this process, we conducted whole-genome sequencing and successfully identified the pivotal 3-PBA-degrading gene <i>sca</i> (1,230 bp). After <i>sca</i> was expressed in engineered bacteria, a remarkable degradation efficiency was observed, as 20 mg/L 3-PBA was almost completely decomposed within 24 h. The phenol was formed as one of the degradation products. Notably, in addition to their ability to degrade 3-PBA, the resting cells proficiently degraded 4'-HO-3-PBA and 3'-HO-4-PBA. In conclusion, we successfully identified and validated <i>sca</i> as the pivotal enzyme responsible for the efficient degradation","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024","modification":"2026-04-08T15:15:05.315Z","creation":"2025-07-27T03:11:07.963Z"},"accession":"S-EPMC11026547","cross_references":{"pubmed":["38646623"],"doi":["10.3389/fmicb.2024.1361335"]}}